Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,479,155	A→G	V8A (GTC→GCC)	yhfA ←	OsmC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,479,155	0	A	G	100.0%	128.0 / NA	38	V8A (GTC→GCC)	yhfA	OsmC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

ATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACAAAGCG  >  NZ_CP009273/3479025‑3479289                                                                                                                                   |                                                                                                                                       aTCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACtt                                                                                                                                 >  1:194564/1‑138 (MQ=255)     aTGGCACTGCAACCACCCGCCGCCATCAACACCATTTCAATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTc                                                                                                                            >  1:276515/1‑139 (MQ=255)      tGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTcg                                                                                                                           >  1:342434/1‑139 (MQ=255)        gCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTcgcg                                                                                                                         >  1:162849/1‑139 (MQ=255)          aCTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGCGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCt                                                                                                                       >  1:66548/1‑139 (MQ=255)                  caccCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCAtttt                                                                                                               <  2:321050/139‑1 (MQ=255)                    cccGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                             >  1:133220/1‑139 (MQ=255)                    cccGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTg                                                                                                             <  1:34540/139‑1 (MQ=255)                                     cATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGACCAATAAAATCTGATGACCAGAGGCGTATTCGCCCAGCAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTTCTACTCCACTGCGTCaa                                                                                            >  2:172732/1‑139 (MQ=255)                                      aTTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAAt                                                                                           <  1:436771/139‑1 (MQ=255)                                             tCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGcc                                                                                                                                      <  1:196144/89‑1 (MQ=255)                                             tCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGcc                                                                                                                                      >  2:196144/1‑89 (MQ=255)                                             tCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTg                                                                                    <  1:561131/139‑1 (MQ=255)                                               ggACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGa                                                                                   <  2:194564/138‑1 (MQ=255)                                                 aCTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACag                                                                                <  2:342434/139‑1 (MQ=255)                                                 aCTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACag                                                                                <  2:162849/139‑1 (MQ=255)                                                     ggTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTa                                                                            >  1:10522/1‑139 (MQ=255)                                                            tATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTAc                                                                     >  2:133857/1‑139 (MQ=255)                                                             aTCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTAc                                                                     >  1:460/1‑138 (MQ=255)                                                              tCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACt                                                                    >  2:334320/1‑138 (MQ=255)                                                              tCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACt                                                                    >  2:238613/1‑138 (MQ=255)                                                                 ccTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAAc                                                                 >  1:471828/1‑138 (MQ=255)                                                                 ccTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCC‑TCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAAcc                                                                >  1:561179/1‑138 (MQ=255)                                                                     aGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAAt                                                            >  1:62693/1‑139 (MQ=255)                                                                     aGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAAt                                                            >  1:90586/1‑139 (MQ=255)                                                                            gTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACag                                                                                >  1:365588/1‑112 (MQ=255)                                                                            gTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACag                                                                                <  2:365588/112‑1 (MQ=255)                                                                            gTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCaa                                                     >  1:477343/1‑139 (MQ=255)                                                                                 ttAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACgg                                                  >  1:46617/1‑137 (MQ=255)                                                                                   aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                               <  2:447432/138‑1 (MQ=255)                                                                                   aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                               <  2:68321/138‑1 (MQ=255)                                                                                   aaaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAg                                               <  1:314016/138‑1 (MQ=255)                                                                                    aaaTCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAgg                                              <  2:567523/138‑1 (MQ=255)                                                                                           atgaCCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGAc                                          <  2:460/135‑1 (MQ=255)                                                                                                         aTTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCg                        <  1:238613/139‑1 (MQ=255)                                                                                                         aTTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCg                        <  1:329536/139‑1 (MQ=255)                                                                                                         aTTCGCCCAGAAAAGTTAACCCTTCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCg                        <  1:334320/139‑1 (MQ=255)                                                                                                                                tCGGCCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACAAAGCg  >  1:25550/1‑138 (MQ=255)                                                                                                                                   |                                                                                                                                       ATCGATGGCACTGCAACCACCCGCCGCCATCAACACCATTTCCATCGGACTTGGTGCTTTATCGCCTGAGTTGCCGTCCATTAAAATCTGATGACCAGAGGCGGATTCGCCCAGAAAAGTTAACCCTTCGACCCACTTCACTCGCGCTTGCATTTTTGCTACTCCACTGCGTCAATTTTCCTGACAGAGTACGCGTACTAACCAAATCGCGCAACGGAAGGCGACCTGGGTCATGCTGAAGCGAGACACCAGGAGACACAAAGCG  >  NZ_CP009273/3479025‑3479289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑