Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,526,544	C→T	A123V (GCT→GTT)	pckA →	phosphoenolpyruvate carboxykinase (ATP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,526,544	0	C	T	100.0%	76.4 / NA	21	A123V (GCT→GTT)	pckA	phosphoenolpyruvate carboxykinase (ATP)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/9);  total (12/9)

CTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGAC  >  NZ_CP009273/3526418‑3526674                                                                                                                               |                                                                                                                                   cTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTgtggtg                                                                                                                         <  2:126591/138‑1 (MQ=255)             cAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCACCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACACGGATAc                                                                                                            >  2:87344/1‑139 (MQ=255)                          aggTAAGAACGACAACAAACCTCTCTCTCCGGCAACCTGGCAGCATCTGAAAGGCCTGGTGACCCGGCAGCTTTCTGGCAAACGTCTGTTCGTTCTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTtccgtc                                                                                               <  2:161259/139‑1 (MQ=255)                                gAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGttt                                                                                          >  1:533958/1‑138 (MQ=255)                                      caacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCg                                                                                                                       >  1:86527/1‑103 (MQ=255)                                      caacaaACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCg                                                                                                                       <  2:86527/103‑1 (MQ=255)                                              ctctctctCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGc                                                                           >  1:330460/1‑139 (MQ=255)                                                        ggAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGgcg                                                                 >  1:342712/1‑139 (MQ=255)                                                                                ccTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTAtt                                         >  1:76378/1‑139 (MQ=255)                                                                                  tGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCg                                       >  1:247980/1‑139 (MQ=255)                                                                                          aGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGc                                >  2:322205/1‑138 (MQ=255)                                                                                           ggCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGaa                                                                                >  1:51731/1‑89 (MQ=255)                                                                                           ggCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGaa                                                                                <  2:51731/89‑1 (MQ=255)                                                                                                 tttCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTtc                                                                                                   <  2:153273/64‑1 (MQ=255)                                                                                                 tttCCGGCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTtc                                                                                                   >  1:153273/1‑64 (MQ=255)                                                                                                       gCAAACGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGc                  <  2:173469/139‑1 (MQ=255)                                                                                                          aaCGTCTGTTCGTTGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGCGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAgg               >  2:245436/1‑139 (MQ=255)                                                                                                                      ttGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCATGTTTCAAACCAGa   <  2:330460/139‑1 (MQ=255)                                                                                                                      ttGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGa   <  2:342712/139‑1 (MQ=255)                                                                                                                      ttGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGa   <  1:322205/139‑1 (MQ=255)                                                                                                                       tGTCGACGTTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGAc  >  2:96472/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                   CTTTCTGGTGGGCAGACAAAGGCAAAGGTAAGAACGACAACAAACCTCTCTCTCCGGAAACCTGGCAGCATCTGAAAGGCCTGGTGACCAGGCAGCTTTCCGGCAAACGTCTGTTCGTTGTCGACGCTTTCTGTGGTGCGAACCCGGATACTCGTCTTTCCGTCCGTTTCATCACCGAAGTGGCCTGGCAGGCGCATTTTGTCAAAAACATGTTTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGAC  >  NZ_CP009273/3526418‑3526674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑