Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,589,221 A→G G181G (GGT→GGC)  livH ← high‑affinity branched‑chain amino acid ABC transporter permease LivH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,589,2210AG100.0% 121.7 / NA 37G181G (GGT→GGC) livHhigh‑affinity branched‑chain amino acid ABC transporter permease LivH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

ACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACT  >  NZ_CP009273/3589092‑3589345
                                                                                                                                 |                                                                                                                            
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                     >  2:302169/1‑139 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                     >  1:134884/1‑139 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                     >  2:260697/1‑139 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                     >  1:391737/1‑139 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCgg                                                                                                                     >  1:339634/1‑139 (MQ=255)
aCCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACCCGCGGCCCATGCgg                                                                                                                     >  1:165931/1‑139 (MQ=255)
                 ccACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAAt                                                                                                    >  2:458775/1‑139 (MQ=255)
                     cgccgcCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTc                                                                                                <  1:158728/139‑1 (MQ=255)
                     cgccgcCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTc                                                                                                <  2:168020/139‑1 (MQ=255)
                                    gcCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCg                                                                                 <  1:302169/139‑1 (MQ=255)
                                    accAATCCCAAAGGTCAGCGCAATCCCCCGGTCGGCGTTAAGGCCAATCAGGCTCGCTCTTTACAGATCTGCCGCGCAGGCACGACCCGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCACCAGCATGGCg                                                                                 <  2:435078/138‑1 (MQ=255)
                                      cAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAg                                                                               <  1:393862/139‑1 (MQ=255)
                                         tCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGaa                                                                            <  2:446748/139‑1 (MQ=255)
                                                ggTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACa                                                                     <  1:536336/139‑1 (MQ=255)
                                                  tCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAAt                                                                   <  1:377071/139‑1 (MQ=255)
                                                      cgcAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATc                                                                  >  1:487038/1‑136 (MQ=255)
                                                           tcccccGGTGGGTGTAAATGCCAAGCAGACTCGCCATTTACAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAACCGTCAGCGCCAGCAGGGCGAGGAAGGTAACAATCCAGATCAc                                                          <  2:339634/136‑1 (MQ=255)
                                                           tCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGTGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCAc                                                          >  2:375258/1‑139 (MQ=255)
                                                              cccGGTCGGTGTTAAGGCCAAGCAGCCTCGCCATTTTCAGATCTCCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGc                                                       <  2:379946/139‑1 (MQ=255)
                                                                         ttAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAtggtg                                             <  1:156933/138‑1 (MQ=255)
                                                                            aTGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTaa                                         >  2:363436/1‑139 (MQ=255)
                                                                             tGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTaa                                         >  1:277169/1‑138 (MQ=255)
                                                                                 aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAg                                                                               <  2:104072/96‑1 (MQ=255)
                                                                                 aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAg                                                                               >  1:104072/1‑96 (MQ=255)
                                                                                 aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGt                                                                         <  1:315529/102‑1 (MQ=255)
                                                                                 aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGt                                                                         >  2:315529/1‑102 (MQ=255)
                                                                                 aaGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATagag                                    >  1:462643/1‑139 (MQ=255)
                                                                                                           ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcc              <  1:128299/135‑1 (MQ=255)
                                                                                                           ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGcc              <  1:458775/135‑1 (MQ=255)
                                                                                                           ccGCGCAGGCACGACACGCGCGGCCCATGCGGGAATAGCAAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAAACCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCccac          <  2:2999/139‑1 (MQ=255)
                                                                                                                   gCACGACACGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACt  <  2:487038/139‑1 (MQ=255)
                                                                                                                         cacGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACa                                                                     <  2:161682/66‑1 (MQ=255)
                                                                                                                         cacGCGCGGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACa                                                                     >  1:161682/1‑66 (MQ=255)
                                                                                                                             cgcgGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                 >  2:347075/1‑82 (MQ=255)
                                                                                                                             cgcgGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                 <  1:56985/82‑1 (MQ=255)
                                                                                                                             cgcgGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                 <  1:347075/82‑1 (MQ=255)
                                                                                                                             cgcgGCCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCAt                                                 >  2:56985/1‑82 (MQ=255)
                                                                                                                                 |                                                                                                                            
ACCGAGCAGCACACCCGCCACCGCCGCCATCGCCGCGCCAATCACAAAGGTCAGCGCAATCACCCGGTCGGTGTTAATGCCAAGCAGACTCGCCATTTTCAGATCTTCCGCGCAGGCACGACACGCGCGACCCATGCGGGAATAGCGAATGAAAATCGTCAGCGCCAGCATGGCGAGGAAGGTAACAATCCAGATCACCGCCTGCATGGTGGTAATAGAGGCAGAGAAGTTTTCGCTATGCCCCACCACCCACT  >  NZ_CP009273/3589092‑3589345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑