Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,617,742	A→G	E2G (GAA→GGA)	yhhI →	ISAs1‑like element ISEc1 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,617,742	0	A	G	100.0%	58.9 / NA	19	E2G (GAA→GGA)	yhhI	ISAs1‑like element ISEc1 family transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

GTTCACTTTTTGCACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGTTGGGA  >  NZ_CP009273/3617613‑3617871                                                                                                                                  |                                                                                                                                  gTTCACTTTTTGCACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTaaaa                                                                                                                           >  1:171318/1‑138 (MQ=255)           tGCACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGa                                                                                                                 <  2:256736/138‑1 (MQ=37)            gCACTAAATAATTCTCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGaa                                                                                                                <  2:53402/138‑1 (MQ=255)                  aataatTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACatat                                                                                                           <  2:125844/137‑1 (MQ=21)                                       aaaaaTTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                     <  1:447324/138‑1 (MQ=21)                                                                        ttttttATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGa                                                     <  1:121298/137‑1 (MQ=37)                                                                        ttttttATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGa                                                     <  1:272933/137‑1 (MQ=37)                                                                                    tataCATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                     >  1:484182/1‑93 (MQ=37)                                                                                    tataCATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGa                                                                                     <  2:484182/93‑1 (MQ=37)                                                                                            aTCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgt                               >  2:574730/1‑139 (MQ=37)                                                                                            aTCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgt                               >  2:511543/1‑139 (MQ=37)                                                                                            aTCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgt                               >  2:15667/1‑139 (MQ=37)                                                                                            aTCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgt                               >  1:461669/1‑139 (MQ=37)                                                                                             tCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgt                               >  1:487103/1‑138 (MQ=37)                                                                                             tCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTtgt                               <  2:487103/138‑1 (MQ=37)                                                                                                         tACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATccc                                                                                               <  2:11952/62‑1 (MQ=25)                                                                                                         tACCTAATTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATccc                                                                                               >  1:11952/1‑62 (MQ=25)                                                                                                               aTTAAGGAGAACAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTg               >  1:462060/1‑136 (MQ=17)                                                                                                                         aCAAAATGGGACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGTTGGGa  <  2:462060/139‑1 (MQ=21)                                                                                                                                  |                                                                                                                                  GTTCACTTTTTGCACTAAATAATTCGCATTTTATGTTTAAAAATTGAGATATTCCTTATTACCTGAAGCTGTTTTTTATTGCTTATACATGATCAAATACTCCTTACCTAATTAAGGAGAACAAAATGGAACTTAAAAAATTGATGGAACATATTTCTATTATCCCCGATTACAGACAAACCTGGAAAGTGGAACATAAATTATCGGATATTCTACTGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGTTGGGA  >  NZ_CP009273/3617613‑3617871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑