Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,836,431	A→G	V240A (GTC→GCC)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,836,431	0	A	G	100.0%	179.6 / NA	50	V240A (GTC→GCC)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

ACCATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGACGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTTA  >  NZ_CP009273/3836295‑3836563                                                                                                                                         |                                                                                                                                     aCCATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    >  1:473775/1‑139 (MQ=255)  ccATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    <  2:473775/138‑1 (MQ=255)  ccATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    <  1:311430/138‑1 (MQ=255)                 gCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCg                                                                                                                    >  1:220717/1‑139 (MQ=255)                  cTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCg                                                                                                                    >  2:13856/1‑138 (MQ=255)                     tATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCggaa                                                                                                                 <  1:58811/138‑1 (MQ=255)                     tATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCgga                                                                                                                  <  2:194019/137‑1 (MQ=255)                           ttACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    >  1:127351/1‑113 (MQ=255)                           ttACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGcg                                                                                                                                    <  2:127351/113‑1 (MQ=255)                             acacCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGa                                                                                                        >  2:159952/1‑139 (MQ=255)                             acacCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGa                                                                                                        >  2:366786/1‑139 (MQ=255)                             acacCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGa                                                                                                        >  2:31894/1‑139 (MQ=255)                              cacCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGAc                                                                                                       <  1:243915/139‑1 (MQ=255)                                   aTTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCgga                                                                                                                  <  2:440968/123‑1 (MQ=255)                                   aTTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCgga                                                                                                                  >  1:440968/1‑123 (MQ=255)                                        tGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTaaaa                                                                                             >  1:191097/1‑139 (MQ=255)                                            ccTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGaa                                                                                         >  1:320354/1‑139 (MQ=255)                                             cTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAgg                                                                                                                      >  2:369241/1‑109 (MQ=255)                                             cTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAgg                                                                                                                      <  1:369241/109‑1 (MQ=255)                                                             gTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGa                                                                                                        <  2:179318/107‑1 (MQ=255)                                                             gTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGa                                                                                                        >  1:179318/1‑107 (MQ=255)                                                             gTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAaataat                                                                        >  2:122731/1‑139 (MQ=255)                                                                      cATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGc                                                                 >  2:421635/1‑137 (MQ=255)                                                                      cATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCaa                                                               <  2:220717/139‑1 (MQ=255)                                                                           aGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAgg                                                                                                                      <  2:526551/79‑1 (MQ=255)                                                                           aGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAgg                                                                                                                      >  1:526551/1‑79 (MQ=255)                                                                           aGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGt                                                          >  2:568803/1‑139 (MQ=255)                                                                              agaAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       <  1:421635/139‑1 (MQ=255)                                                                                aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCt                                                                                                 <  1:373954/95‑1 (MQ=255)                                                                                aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCt                                                                                                 >  2:373954/1‑95 (MQ=255)                                                                                aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACAt                                                                                      <  2:447338/106‑1 (MQ=255)                                                                                aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACAt                                                                                      >  1:447338/1‑106 (MQ=255)                                                                                aaaaTGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcacca                                                     >  2:412808/1‑139 (MQ=255)                                                                                      aTACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATa                                                <  2:211940/138‑1 (MQ=255)                                                                                                       tgtTAACGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCa                              <  1:221533/139‑1 (MQ=255)                                                                                                           aaCGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGa                           >  2:164578/1‑138 (MQ=255)                                                                                                           aaCGCGCCGCTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGa                           >  1:77291/1‑138 (MQ=255)                                                                                                                 ccgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGaa                                                                               >  1:473333/1‑80 (MQ=255)                                                                                                                 ccgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGaa                                                                               <  2:473333/80‑1 (MQ=255)                                                                                                                 ccgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGAc                    <  1:562542/139‑1 (MQ=255)                                                                                                                 ccgcTCAAATCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGAc                    <  1:169894/139‑1 (MQ=255)                                                                                                                       aaaTCTACTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGtga                <  2:379852/137‑1 (MQ=255)                                                                                                                       aaaTCTACTTCACCAATGGCGCCGCTAATAGCAGGCGGAATGGAATAGACGCCGCTAAAAGGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGtga                <  2:267629/137‑1 (MQ=255)                                                                                                                              cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCCCCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGacaac         <  2:320354/137‑1 (MQ=255)                                                                                                                              cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGacaa          <  2:528936/136‑1 (MQ=255)                                                                                                                              cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAacac       >  1:469993/1‑139 (MQ=255)                                                                                                                              cTTCACCAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAacac       >  1:266489/1‑139 (MQ=255)                                                                                                                                   ccAATGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTta  <  1:308119/139‑1 (MQ=255)                                                                                                                                      aTGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       <  1:466661/83‑1 (MQ=255)                                                                                                                                      aTGGCGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTcac                                                       >  2:466661/1‑83 (MQ=255)                                                                                                                                         |                                                                                                                                     ACCATCTATTAAGCCCGCTTTATCAGTTACACCAATTAATGTTCCTGATGAATCAAATAGGTTGATCAGCATAAAGGAGAAAATGATACCGGCGAGTTCAAGTGTTAACGCGCCGCTCAAATCTACTTCACCAATGACGCCGCTAATATCAGGCGGAATGGAATAGACGCCGCTAAAATGAACATCACCGAAAAATAATCCACAGCAAGACGTCACCACAATAGAAACCAGCACCGCGGCATGAAAATGACGTGATGACAACACGGTTA  >  NZ_CP009273/3836295‑3836563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑