Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,841,593	A→G	F131S (TTC→TCC)	uhpC ←	MFS transporter family glucose‑6‑phosphate receptor UhpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,841,593	0	A	G	100.0%	107.9 / NA	31	F131S (TTC→TCC)	uhpC	MFS transporter family glucose‑6‑phosphate receptor UhpC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/13);  total (18/13)

CCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGCTGAC  >  NZ_CP009273/3841462‑3841723                                                                                                                                    |                                                                                                                                   ccATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAgg                                                                                                                              <  1:215781/138‑1 (MQ=255)            ggAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCAcc                                                                                                                   <  1:276176/138‑1 (MQ=255)                  aGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCa                                                                                                            >  2:244427/1‑139 (MQ=255)                    tGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACg                                                                                                          >  2:36596/1‑139 (MQ=255)                      cgccgcCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACgg                                                                                                         >  2:426700/1‑138 (MQ=255)                      cgccgcCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACgg                                                                                                         <  1:455666/138‑1 (MQ=255)                                             ttCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCgag                                                                                  <  1:286608/138‑1 (MQ=255)                                                     tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCAcc                                                                                                                   <  2:535934/97‑1 (MQ=255)                                                     tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCAcc                                                                                                                   >  1:535934/1‑97 (MQ=255)                                                     tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCaaa                                                                          >  1:220414/1‑138 (MQ=255)                                                     tGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAAc                                                                         <  1:36596/139‑1 (MQ=255)                                                          accaACCGCCGCGCTCTGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAATTGCGTTCAGCACCCATAGCACGCCAAACGCCCCTAGCGACGTCGAGAAGCCAAACAGAAt                                                                    >  1:286012/1‑139 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                    >  1:512230/1‑139 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                    >  2:51225/1‑139 (MQ=255)                                                          accaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                    >  2:174059/1‑139 (MQ=255)                                                                               gTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGAt                                               <  2:406032/139‑1 (MQ=255)                                                                                      ccAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGccc                                         >  2:479711/1‑138 (MQ=255)                                                                                      ccAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGccc                                         >  1:444223/1‑138 (MQ=255)                                                                                          gCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATcc                                    >  1:76062/1‑139 (MQ=255)                                                                                           ccGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                    >  2:196121/1‑106 (MQ=255)                                                                                           ccGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAt                                                                    <  1:196121/106‑1 (MQ=255)                                                                                                        cgcACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATa                                              <  1:33317/115‑1 (MQ=255)                                                                                                        cgcACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATa                                              >  2:33317/1‑115 (MQ=255)                                                                                                        cgcACACACCGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACgg                      >  2:191616/1‑139 (MQ=255)                                                                                                                 cGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAg             <  1:51225/139‑1 (MQ=255)                                                                                                                 cGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAg             <  1:244427/139‑1 (MQ=255)                                                                                                                 cGGTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAg             <  1:174059/139‑1 (MQ=255)                                                                                                                   gTGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGc            >  1:139955/1‑138 (MQ=255)                                                                                                                    tGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGa          >  2:36454/1‑139 (MQ=255)                                                                                                                    tGAACCCCAGCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAAGGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGa          <  2:358808/139‑1 (MQ=255)                                                                                                                             gCCCTGGGAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGCTGAc  >  1:3080/1‑138 (MQ=255)                                                                                                                                    |                                                                                                                                   CCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACCGGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCCATAGCGACGTCGAGAAGCCAAACAGAATGTTGATAATGCCCGTGGCGATAAGCCCTATCCCCATAAAATAACGGGCATTTGAGCGATCGCTGAC  >  NZ_CP009273/3841462‑3841723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑