Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,853,103	A→G	F125S (TTC→TCC)	yidK ←	solute:sodium symporter family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,853,103	0	A	G	100.0%	140.2 / NA	39	F125S (TTC→TCC)	yidK	solute:sodium symporter family transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/22);  total (17/22)

ACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAG  >  NZ_CP009273/3852977‑3853235                                                                                                                               |                                                                                                                                     aCCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCg                                                                                                                           <  1:238880/138‑1 (MQ=255)        gCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGataa                                                                                                                    <  2:380349/138‑1 (MQ=255)         ccAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGataata                                                                                                                  <  1:517659/139‑1 (MQ=255)         ccAGATAGCCGCACCGTGAGAAATCTGTAGCGATGCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCTGAGTAGAGAACAATCGGCAGAAAGCAGCCGCCGGTGGCAATTAGGGAGCAGAAGTCGATGataata                                                                                                                  <  2:214277/139‑1 (MQ=255)             aTAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgcg                                                                                                              <  2:250351/139‑1 (MQ=255)             aTAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATAcgcg                                                                                                              <  1:72087/139‑1 (MQ=255)                  cGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGtt                                                                                                         <  2:527557/139‑1 (MQ=255)                    cACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGtttt                                                                                                       <  2:371755/139‑1 (MQ=255)                        gTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCa                                                                                                   <  2:160360/139‑1 (MQ=255)                         tGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAg                                                                                                                                    >  2:4534/1‑105 (MQ=255)                         tGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAg                                                                                                                                    <  1:4534/105‑1 (MQ=255)                                   gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                        >  2:496495/1‑139 (MQ=255)                                   gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                        >  2:406153/1‑139 (MQ=255)                                   gTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTtcctc                                                                                        >  1:569236/1‑139 (MQ=255)                                        gATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGa                                                                                    >  1:380615/1‑138 (MQ=255)                                            ccccGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATc                                                                               >  2:5352/1‑139 (MQ=255)                                            ccccGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATc                                                                               >  1:273290/1‑139 (MQ=255)                                             cccGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCg                                                                              <  1:439264/139‑1 (MQ=255)                                                  cGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGAt                                                                         <  2:532785/139‑1 (MQ=255)                                                  cGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGAt                                                                         <  1:2873/139‑1 (MQ=255)                                                      aaaCAGGCTGTTTAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAGTCGGGGAtttgg                                                                     >  2:245485/1‑135 (MQ=255)                                                            gCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATg                                                               >  2:204379/1‑139 (MQ=255)                                                                        cAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAg                                                                                                                                 >  1:12884/1‑61 (MQ=255)                                                                        cAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAg                                                                                                                                 <  2:12884/61‑1 (MQ=255)                                                                             cgcCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAgcg                                              <  2:273290/139‑1 (MQ=255)                                                                               ccGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGcgcg                                            >  2:149864/1‑139 (MQ=255)                                                                                      agagAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                             >  2:284165/1‑115 (MQ=255)                                                                                      agagAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                             <  1:284165/115‑1 (MQ=255)                                                                                            cAATCGGCAGAAAGCAGACTCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAg                               >  2:243844/1‑139 (MQ=255)                                                                                               tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCggg                                                                            <  2:352449/91‑1 (MQ=255)                                                                                               tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCggg                                                                            >  1:352449/1‑91 (MQ=255)                                                                                               tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                             >  1:154996/1‑106 (MQ=255)                                                                                               tCGGCAGAAAGCAGACGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGcc                                                             <  2:154996/106‑1 (MQ=255)                                                                                                    aGAAAGCAGACGCCGGGGGCAACTAGGGCGCAGAAGTCGATGATAATACGCGTCGCTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGCGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAgg                        >  1:75910/1‑138 (MQ=255)                                                                                                              cGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGt             <  2:224529/139‑1 (MQ=255)                                                                                                              cGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGt             <  1:496495/139‑1 (MQ=255)                                                                                                              cGCCGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGt             <  1:204379/139‑1 (MQ=255)                                                                                                                ccGGTGGCAATTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCa           <  2:285070/139‑1 (MQ=255)                                                                                                                         aTTAGGGAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAg  >  2:217945/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                     ACCAGTAGCCAGATAGCCGCACCGTGAGAAATCTGTAGCGATTCCCCGACGTGAAACAGGCTGTTGAGCGCCAACGCGCCGGAGTAGAGAACAATCGGCAGAAAGCAGACGCCGGTGGCAATTAGGAAGCAGAAGTCGATGATAATACGCGTCGTTTTATCATAACGTTCCTCCAGAAAATCGGGGATGGTGGCAATGCCGCGCTTCAGATAGCGCGGTAAAAAGATTAGCGCGAGGAAGATCAGCGTCACCGCTGAAG  >  NZ_CP009273/3852977‑3853235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑