Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,873,583	A→G	S333S (AGT→AGC)	recF ←	DNA replication/repair protein RecF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,873,583	0	A	G	100.0%	131.7 / NA	37	S333S (AGT→AGC)	recF	DNA replication/repair protein RecF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/22);  total (15/22)

TGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTG  >  NZ_CP009273/3873449‑3873715                                                                                                                                       |                                                                                                                                     tGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCt                                                                                                                                    <  1:122737/137‑1 (MQ=255)      cTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATcgcg                                                                                                                             <  1:552485/139‑1 (MQ=255)             agTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACaa                                                                                                                      <  1:317173/139‑1 (MQ=255)              gTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACaaa                                                                                                                     <  1:130978/139‑1 (MQ=255)              gTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACa                                                                                                                       >  1:315729/1‑137 (MQ=255)              gTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACa                                                                                                                       >  2:165069/1‑137 (MQ=255)                 aTAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATcgcg                                                                                                                             >  2:403229/1‑128 (MQ=255)                 aTAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATcgcg                                                                                                                             <  1:403229/128‑1 (MQ=255)                             cATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGt                                                                                                      <  2:347165/139‑1 (MQ=255)                             cATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGt                                                                                                      <  1:546234/139‑1 (MQ=255)                                    gTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCtttt                                                                                               <  1:304751/139‑1 (MQ=255)                                           gCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGc                                                                                           <  2:315729/136‑1 (MQ=255)                                                         gggTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCcg                                                                          >  2:569679/1‑139 (MQ=255)                                                         gggTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCcg                                                                          >  1:472384/1‑139 (MQ=255)                                                                tCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCt                                                                   <  1:569679/139‑1 (MQ=255)                                                                  cGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCtca                                                                 <  2:54073/139‑1 (MQ=255)                                                                  cGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCtca                                                                 <  1:52384/139‑1 (MQ=255)                                                                      aTTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCt                                                                   <  1:292309/133‑1 (MQ=255)                                                                      aTTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCt                                                                   >  2:292309/1‑133 (MQ=255)                                                                            cccTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCt                                                       <  2:248040/139‑1 (MQ=255)                                                                            cccTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCt                                                       >  1:304585/1‑139 (MQ=255)                                                                                           aaCATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAatcat                                        >  1:199563/1‑139 (MQ=255)                                                                                                   cGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    <  2:51331/135‑1 (MQ=255)                                                                                                   cGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    <  2:29552/135‑1 (MQ=255)                                                                                                   cGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    <  1:146506/135‑1 (MQ=255)                                                                                                                            aCGTGTTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCa        <  2:420647/138‑1 (MQ=255)                                                                                                                               tgtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACggg    >  1:449386/1‑139 (MQ=255)                                                                                                                               tgtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACggg    >  1:132012/1‑139 (MQ=255)                                                                                                                                gtTCAGCGCTGATCGCGCTGACAAGGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGt   >  2:395317/1‑139 (MQ=255)                                                                                                                                gtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    >  1:425054/1‑106 (MQ=255)                                                                                                                                gtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    >  1:292982/1‑106 (MQ=255)                                                                                                                                gtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    <  2:425054/106‑1 (MQ=255)                                                                                                                                gtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTAt                                    <  2:292982/106‑1 (MQ=255)                                                                                                                                gtTCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGt   >  1:445223/1‑139 (MQ=255)                                                                                                                                 ttCAGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTg  <  2:304585/139‑1 (MQ=255)                                                                                                                                    aGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGccgccc               <  2:69372/123‑1 (MQ=255)                                                                                                                                    aGCGCTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGccgccc               >  1:69372/1‑123 (MQ=255)                                                                                                                                       |                                                                                                                                     TGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTAATCCGTTATTTTACCCTTTTCCACGGTAAACATCTTCGAATTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTG  >  NZ_CP009273/3873449‑3873715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑