Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,877,538	A→G	intergenic (+72/‑158)	BW25113_RS25780 → / → rpmH	protein YsdD/50S ribosomal protein L34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,877,538	0	A	G	100.0%	159.3 / NA	45	intergenic (+72/‑158)	BW25113_RS25780/rpmH	protein YsdD/50S ribosomal protein L34
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/20);  total (25/20)

TCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAT  >  NZ_CP009273/3877416‑3877673                                                                                                                           |                                                                                                                                        tCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAAt                                                                                                                           >  2:258762/1‑137 (MQ=255)      taaAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGaaa                                                                                                                    <  2:440130/139‑1 (MQ=255)           aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGaa                                                                                                               >  2:392486/1‑139 (MQ=255)           aTCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGaa                                                                                                               >  1:518559/1‑139 (MQ=255)            tCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAAt                                                                                                              <  1:131741/139‑1 (MQ=255)               aGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGa                                                                                                           >  2:508650/1‑139 (MQ=255)                    gATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCg                                                                                                      <  1:508650/139‑1 (MQ=255)                     aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAAt                                                                                                              >  1:139285/1‑130 (MQ=255)                     aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAAt                                                                                                              <  2:139285/130‑1 (MQ=255)                     aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTcc                                                                                                       >  1:20643/1‑137 (MQ=255)                     aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTcc                                                                                                       <  2:20643/137‑1 (MQ=255)                     aTCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCg                                                                                                      >  2:467451/1‑138 (MQ=255)                           gcgcTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAgtgt                                                                                                <  1:258762/138‑1 (MQ=255)                           gcgcTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAgtgt                                                                                                <  1:381352/138‑1 (MQ=255)                           gcgcTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAgtgt                                                                                                <  1:435923/138‑1 (MQ=255)                                 tACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAat                                                                                           <  1:110698/137‑1 (MQ=255)                                   cccATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAattat                                                                                        >  2:336657/1‑138 (MQ=255)                                      aTCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTAc                                                                                     >  1:108829/1‑138 (MQ=255)                                            ccGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCgg                                                                              <  1:277610/139‑1 (MQ=255)                                                         cACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAc                                                                 >  1:297135/1‑139 (MQ=255)                                                           cccGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCAcc                                                                >  1:514970/1‑138 (MQ=255)                                                              ggCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATg                                                            >  2:451536/1‑139 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTaaa                                                                                                                            >  1:281206/1‑73 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTaaa                                                                                                                            <  2:281206/73‑1 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAAt                                                                                  >  1:306050/1‑115 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAAt                                                                                  <  2:306050/115‑1 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGc                                                          >  2:441410/1‑139 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGc                                                          >  2:538640/1‑139 (MQ=255)                                                                cgcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGc                                                          >  1:219702/1‑139 (MQ=255)                                                                 gcgcCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGTGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCt                                                         <  1:161155/139‑1 (MQ=255)                                                                    ccATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCg                                                      <  1:9018/139‑1 (MQ=255)                                                                                 cACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGttt                                         >  1:175244/1‑139 (MQ=255)                                                                                         tgAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCAttttt                                  <  2:175244/138‑1 (MQ=255)                                                                                           aGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTcgg                               >  1:57196/1‑139 (MQ=255)                                                                                           aGGTCGTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTcgg                               <  1:441410/139‑1 (MQ=255)                                                                                                gTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCg                                                      >  1:435825/1‑111 (MQ=255)                                                                                                gTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCg                                                      <  2:435825/111‑1 (MQ=255)                                                                                                gTACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGa                           >  2:431905/1‑138 (MQ=255)                                                                                                 tACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                         <  1:392486/139‑1 (MQ=255)                                                                                                 tACATTTTCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGata                         >  2:381415/1‑139 (MQ=255)                                                                                                     ttttCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATcc                      >  1:178769/1‑138 (MQ=255)                                                                                                       ttCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCaa                    <  2:57196/138‑1 (MQ=255)                                                                                                        tCCCTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAAt                   >  2:154065/1‑138 (MQ=255)                                                                                                         cccTGCGAAAAGGTGCGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATa                  <  1:508556/138‑1 (MQ=255)                                                                                                                        cGGGAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAt  >  2:101540/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                        TCTAATAAAGATCCAGGACGATCCTTGCGCTTTACCCATCAGCCCGTATAATCCTCCACCCGGCGCGCCATGCTGGTTTCCACTGGTGTGAGGTCGTACATTTTCCCTGCGAAAAGGTGCGGAAAAGCGCGGTAAATAAGGAAAGAGAATTGACTCCGGAGTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTAT  >  NZ_CP009273/3877416‑3877673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑