BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,882,139	A→G	E17G (GAG→GGG)	tnaA →	tryptophanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,882,139	0	A	G	100.0%	134.5 / NA	37	E17G (GAG→GGG)	tnaA	tryptophanase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/12); total (25/12)

TGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAG  >  NZ_CP009273/3882016‑3882272

tGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTAc                                                                                                                        >  2:531754/1‑139 (MQ=255)

 ggCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTAc                                                                                                                        >  2:560436/1‑138 (MQ=255)

                ataGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGt                                                                                                        >  2:488045/1‑139 (MQ=255)

                         gTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACCTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGc                                                                                                >  1:99655/1‑138 (MQ=255)

                                  acaGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTaaa                                                                                      >  2:497204/1‑139 (MQ=255)

                                    aGGGCTAACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGATTATCGTGACGAGGCACTTATTAAATc                                                                                    >  2:365005/1‑139 (MQ=255)

                                    aGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGCGGCAATTATTAAATc                                                                                    >  2:499101/1‑139 (MQ=255)

                                    aGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATc                                                                                    >  2:287377/1‑139 (MQ=255)

                                    aGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATc                                                                                    >  2:223298/1‑139 (MQ=255)

                                         tCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTa                                                                               >  2:51336/1‑139 (MQ=255)

                                           aCTGTAATTAAAATAAATGGAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTAt                                                                              >  1:501959/1‑138 (MQ=255)

                                               tAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAAc                                                                          <  1:499101/138‑1 (MQ=255)

                                                  ttAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGt                                                                      >  1:121512/1‑139 (MQ=255)

                                                       ataaatGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACt                                                                                                                    >  1:176137/1‑88 (MQ=255)

                                                       ataaatGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACt                                                                                                                    <  2:176137/88‑1 (MQ=255)

                                                       ataaatGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg                                                                 >  1:365918/1‑139 (MQ=255)

                                                                           tGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGtttt                                             >  1:84464/1‑139 (MQ=255)

                                                                                aaCTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATc                                         >  1:22942/1‑138 (MQ=255)

                                                                                      aaaCATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTa                                   <  1:488045/138‑1 (MQ=255)

                                                                                      aaaCATCTCCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTa                                   <  1:51336/138‑1 (MQ=255)

                                                                                            ctcCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGAcc                             >  1:9837/1‑138 (MQ=255)

                                                                                            ctcCCTGAACCGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGAcc                             >  1:399642/1‑138 (MQ=255)

                                                                                                      cGTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCAc                  >  1:159754/1‑139 (MQ=255)

                                                                                                       gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg                                                                 >  1:12119/1‑91 (MQ=255)

                                                                                                       gTTCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg                                                                 <  2:12119/91‑1 (MQ=255)

                                                                                                        ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGCAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                >  2:227418/1‑139 (MQ=255)

                                                                                                        ttCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCg                >  2:234615/1‑139 (MQ=255)

                                                                                                         tCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg                                                                 <  2:131282/89‑1 (MQ=255)

                                                                                                         tCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCctg                                                                 >  1:131282/1‑89 (MQ=255)

                                                                                                         tCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCAc                  <  1:543960/136‑1 (MQ=255)

                                                                                                         tCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCAc                  >  2:543960/1‑136 (MQ=255)

                                                                                                         tCCGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCgg               >  1:532428/1‑139 (MQ=255)

                                                                                                           cGCATTCGTGTTATTGGTCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCgggg             <  1:287377/139‑1 (MQ=255)

                                                                                                           cGCATTCGTGTTATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCgggg             <  1:560436/139‑1 (MQ=255)

                                                                                                                     ttATTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGc    <  2:532428/138‑1 (MQ=255)

                                                                                                                       aTTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCag  <  1:223298/138‑1 (MQ=255)

                                                                                                                       aTTGGGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCag  <  2:501959/138‑1 (MQ=255)

TGGCGAATTAATCGGTATAGCAGATGTAATATTCACAGGGATCACTGTAATTAAAATAAATGAAGGATTATGTAATGGAAAACTTTAAACATCTCCCTGAACCGTTCCGCATTCGTGTTATTGAGCCAGTAAAACGTACCACTCGCGCTTATCGTGAAGAGGCAATTATTAAATCCGGTATGAACCCGTTCCTGCTGGATAGCGAAGATGTTTTTATCGATTTACTGACCGACAGCGGCACCGGGGCGGTGACGCAG  >  NZ_CP009273/3882016‑3882272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`