BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,219	A→G	T281T (ACT→ACC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,219	0	A	G	100.0%	128.1 / NA	36	T281T (ACT→ACC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/17); total (19/17)

CGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCAGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGCAACATATCCTG  >  NZ_CP009273/3896084‑3896347

cGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGt                                                                                                                                <  2:133643/138‑1 (MQ=255)

cGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGt                                                                                                                                <  1:403486/138‑1 (MQ=255)

  gATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCAt                                                                                                                             <  1:153430/139‑1 (MQ=255)

  gATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCAt                                                                                                                             >  2:511705/1‑139 (MQ=255)

       gggATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCaa                                                                                                                        >  1:348645/1‑139 (MQ=255)

                        gTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCaa                                                                                                                        >  2:296381/1‑122 (MQ=255)

                        gTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCaa                                                                                                                        <  1:296381/122‑1 (MQ=255)

                          tGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcgcg                                                                                                     >  1:46132/1‑139 (MQ=255)

                          tGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTcgcg                                                                                                     >  2:434648/1‑139 (MQ=255)

                            cccTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCgg                                                                                                    >  2:462053/1‑138 (MQ=255)

                               tGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGgaag                                                                                                 >  1:317152/1‑138 (MQ=255)

                               tGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGgaag                                                                                                 >  1:391276/1‑138 (MQ=255)

                                 cgcATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGaaa                                                                                              <  2:391276/139‑1 (MQ=255)

                                   cATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGGCATCTCAATGGTGATATTGTGGTCGCGGAAGAAAct                                                                                            <  2:41700/139‑2 (MQ=255)

                                                                  acaACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGcc                                                             <  1:187830/139‑1 (MQ=255)

                                                                      ccAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGaa                                                         >  2:574965/1‑139 (MQ=255)

                                                                         gTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACAt                                                      <  1:122268/139‑1 (MQ=255)

                                                                           cATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCa                                                    >  1:239129/1‑139 (MQ=255)

                                                                            aTGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCa                                                    <  1:147363/138‑1 (MQ=255)

                                                                             tGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCAcc                                                  <  2:46132/139‑1 (MQ=255)

                                                                                    aCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGc                                                                                 <  2:302043/101‑1 (MQ=255)

                                                                                    aCTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGc                                                                                 >  1:302043/1‑101 (MQ=255)

                                                                                              aTGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGcc                                 >  2:522311/1‑139 (MQ=255)

                                                                                              aTGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGcc                                 >  2:514863/1‑139 (MQ=255)

                                                                                              aTGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGcc                                 >  1:311985/1‑139 (MQ=255)

                                                                                                 aaaTCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCgg                               <  1:217680/138‑1 (MQ=255)

                                                                                                        cGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAcg                                                                 <  1:42991/97‑1 (MQ=255)

                                                                                                        cGGTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCAcg                                                                 >  2:42991/1‑97 (MQ=255)

                                                                                                         ggTATGTTTTAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTcc                      <  2:270021/139‑1 (MQ=255)

                                                                                                               ttttAAATCTTCTGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGAATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGc                 <  1:541555/138‑1 (MQ=255)

                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGc             >  1:503841/1‑132 (MQ=255)

                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGc             <  2:503841/132‑1 (MQ=255)

                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGCAACatat      >  2:360845/1‑139 (MQ=255)

                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGCAACatat      >  2:541142/1‑139 (MQ=255)

                                                                                                                         tctGCGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGCAACatat      >  2:2628/1‑139 (MQ=255)

                                                                                                                             cGTCACTTTCGGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGCAACATATCCtg  <  2:397874/139‑1 (MQ=255)

CGGATTGGGGATCATATTCAGTATGTTGCCCTGCGCATTTTTATTAATGCTTTCGTCGTGGGAAACACAACCAGTCATGTAATAACTAAAAGAGATGAAATCGACGGTATGTTTTAAATCTTCTGCGTCACTTTCAGTCATCTCAATGGTGATATTGTGGTCGCGGAAGAAACGCTGCATATAGCCGGGATACTGGCCACGCGCCTGAACATCACCAAAGAACATCCAGCGCCGGTTCTCTTCCATGGCCTGCAACATATCCTG  >  NZ_CP009273/3896084‑3896347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`