Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,965,761	A→G	D381G (GAC→GGC)	wecC →	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,965,761	0	A	G	100.0%	117.4 / NA	34	D381G (GAC→GGC)	wecC	UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

CTGCGCGAAAGCCCGGCGATGGAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAAT  >  NZ_CP009273/3965628‑3965892                                                                                                                                      |                                                                                                                                    cTGCGCGAAAGCCCGGCGATGGAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAgg                                                                                                                                >  1:249503/1‑139 (MQ=255)    cgcgAAAGCCCGGCGATGGAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGgcgc                                                                                                                             >  1:362734/1‑139 (MQ=255)          aGCCCGGCGATGGAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCaa                                                                                                                       <  1:99144/139‑1 (MQ=255)                 cGATGGAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGa                                                                                                                <  2:362734/139‑1 (MQ=255)                     ggAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTg                                                                                                            >  1:193817/1‑139 (MQ=255)                      gAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTg                                                                                                            <  1:414459/138‑1 (MQ=255)                        aaTCGCTGCACTTCTCGCCCCGTGGCATAGCGGCGACACTCTGGTTGTTGCGCCTAACATCCCCCAGTTGCCGACAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTg                                                                                                         <  2:205100/139‑1 (MQ=255)                             cTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGAt                                                                                                    >  1:567430/1‑139 (MQ=255)                                     aTCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGt                                                                                              <  2:567430/137‑1 (MQ=255)                                     aTCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGt                                                                                              <  1:506928/137‑1 (MQ=255)                                     aTCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGt                                                                                              <  1:414099/137‑1 (MQ=255)                                     aTCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGt                                                                                              <  1:323935/137‑1 (MQ=255)                                     aTCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                                                             >  2:107211/1‑138 (MQ=255)                                     aTCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTc                                                                                             >  1:44076/1‑138 (MQ=255)                                             gTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCTCCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGt                                                                                    >  2:330173/1‑139 (MQ=255)                                             gTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGt                                                                                    <  2:12763/139‑1 (MQ=255)                                             gTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGt                                                                                    <  1:94122/139‑1 (MQ=255)                                                    aGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTc                                                                              >  1:365462/1‑138 (MQ=255)                                                           aaaCTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTAt                                                                      >  1:231292/1‑139 (MQ=255)                                                                 tGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAAt                                                                  <  2:113686/137‑1 (MQ=255)                                                                       ttgAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACaa                                                          <  2:334354/139‑1 (MQ=255)                                                                          aGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATg                                                        <  2:249503/138‑1 (MQ=255)                                                                           gCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTc                                                      >  1:264947/1‑139 (MQ=255)                                                                                      accaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACaa                                                          <  1:424129/124‑1 (MQ=255)                                                                                      accaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACaa                                                          >  2:424129/1‑124 (MQ=255)                                                                                      accaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTa                                           >  1:2153/1‑139 (MQ=255)                                                                                      accaGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTa                                           >  1:226930/1‑139 (MQ=255)                                                                                        caGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATg                                         >  2:137940/1‑139 (MQ=255)                                                                                              ccGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATgtcgtc                                    <  1:26562/138‑1 (MQ=255)                                                                                                              ggCTTTGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTg                   >  2:367582/1‑139 (MQ=255)                                                                                                                   tGTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGgcg                <  2:264947/137‑1 (MQ=255)                                                                                                                    gTACTCTGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCt              >  2:539991/1‑138 (MQ=255)                                                                                                                         ctGGCGCAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCtgat           >  1:325035/1‑136 (MQ=255)                                                                                                                               cAGCTTGGCGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAAt  >  2:286227/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    CTGCGCGAAAGCCCGGCGATGGAAATCGCTGAACTGATCGCCCAGTGGCATAGCGGCGAAACTCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGCTTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAAGTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTCTGGCGCTGATGAGAAAAAT  >  NZ_CP009273/3965628‑3965892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑