| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,003,978 | A→G | E140G (GAG→GGG) | yigL → | sugar/pyridoxal phosphate phosphatase YigL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,003,978 | 0 | A | G | 96.5% | 99.1 / ‑6.4 | 29 | E140G (GAG→GGG) | yigL | sugar/pyridoxal phosphate phosphatase YigL |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (16/12); minor base T (0/1); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAACGACAATCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGAGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTG > NZ_CP009273/4003843‑4004111 | tCAACGACAATCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGcc > 2:96223/1‑139 (MQ=255) tCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGCGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGt > 1:454043/1‑138 (MQ=255) tCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGt > 2:523414/1‑138 (MQ=255) aCGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCAc > 2:112448/1‑139 (MQ=255) tttATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTg < 2:296222/139‑1 (MQ=255) tCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGa < 1:465124/138‑1 (MQ=255) tATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAActgct < 1:96223/138‑1 (MQ=255) gccatcgccCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGATg > 2:36500/1‑137 (MQ=255) agagATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAAc < 1:347582/139‑1 (MQ=255) aTGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGc > 2:88062/1‑118 (MQ=255) aTGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGc < 1:88062/118‑1 (MQ=255) tGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGTGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCg < 1:420605/139‑1 (MQ=255) cTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTgg > 2:569077/1‑139 (MQ=255) ttCCAATATGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTc < 1:434960/138‑1 (MQ=255) tatGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTtct < 1:523414/139‑1 (MQ=255) tGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACg < 1:218026/104‑1 (MQ=255) tGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACg > 2:218026/1‑104 (MQ=255) tGCGCTGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTtctc > 1:406939/1‑138 (MQ=255) tGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCt > 1:415548/1‑138 (MQ=255) tGTATGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCt > 1:384965/1‑138 (MQ=255) tGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAAcc < 1:290201/139‑1 (MQ=255) tGAGCCTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAAc < 2:406939/138‑1 (MQ=255) ccTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCACCGTCAGTTTCTCTACCTTAACCTGTc > 2:94968/1‑139 (MQ=255) ccTGGATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTc > 2:456748/1‑139 (MQ=255) gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTgg > 1:216922/1‑138 (MQ=255) gATTACTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTgg > 1:35909/1‑138 (MQ=255) cTGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTg < 1:79772/138‑1 (MQ=255) tGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGAt > 2:205016/1‑95 (MQ=255) tGGGGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGAt < 1:205016/95‑1 (MQ=255) | TCAACGACAATCCGGACATCATTACTAACGTTTATCGCGACGACGAATGGTTTATGAATCGCCATCGCCCGGAAGAGATGCGCTTTTTTAAAGAAGCGGTGTTCCAATATGCGCTGTATGAGCCTGGATTACTGGAGCCGGAAGGCGTCAGCAAAGTGTTCTTCACCTGCGATTCCCATGAACAACTGCTGCCGCTGGAGCAGGCGATTAACGCTCGTTGGGGCGATCGCGTCAACGTCAGTTTCTCTACCTTAACCTGTCTGGAAGTG > NZ_CP009273/4003843‑4004111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |