Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,025,290	A→C	D257A (GAC→GCC)	pepQ →	Xaa‑Pro dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,025,290	0	A	C	100.0%	69.2 / NA	20	D257A (GAC→GCC)	pepQ	Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/8);  total (12/8)

GTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGT  >  NZ_CP009273/4025154‑4025421                                                                                                                                         |                                                                                                                                    gTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGctgccc                                                                                                                                   <  2:430940/139‑1 (MQ=255)  tACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGctgccct                                                                                                                                  <  2:509813/139‑1 (MQ=255)        aCAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCtgacctgacc                                                                                                                              <  2:474208/137‑1 (MQ=255)                                  cacGCTGCGGTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAAc                                                                                                   >  2:178248/1‑138 (MQ=255)                                           gTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACgcgc                                                                                         <  1:154200/139‑1 (MQ=255)                                           gTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACgcgc                                                                                         <  2:487923/139‑1 (MQ=255)                                                   ttACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGt                                                                                  >  2:250777/1‑138 (MQ=255)                                                   ttACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGt                                                                                  >  1:368572/1‑138 (MQ=255)                                                         cAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGAc                                                                           >  1:304055/1‑139 (MQ=255)                                                           aaCTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGt                                                                         >  1:74754/1‑139 (MQ=255)                                                           aaCTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGt                                                                         >  2:341697/1‑139 (MQ=255)                                                           aaCTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGt                                                                         >  2:507865/1‑139 (MQ=255)                                                                 aCCATCAGGCATCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGTCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAatga                                                                   <  1:389551/139‑1 (MQ=255)                                                                           aCCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTg                                                         >  1:465607/1‑139 (MQ=255)                                                                             cGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGgc                                                       >  2:46830/1‑139 (MQ=255)                                                                                           aGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGccacca                                         <  1:366158/139‑1 (MQ=255)                                                                                                    ctgGATGCCGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCa                                 <  1:377258/138‑1 (MQ=255)                                                                                                           ccGGGGCAGAATATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAg                         >  1:418426/1‑139 (MQ=255)                                                                                                                    aaTATAACGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGa                <  1:46830/139‑1 (MQ=255)                                                                                                                          aCGGCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTAcc           <  2:74754/138‑1 (MQ=255)                                                                                                                             gCTATGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACAt       <  1:507865/139‑1 (MQ=255)                                                                                                                                aTGCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATc      >  1:227213/1‑137 (MQ=255)                                                                                                                                  gCGGCTGCCCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGt  >  1:564281/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    GTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACACCAAACTGGACCATCAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGT  >  NZ_CP009273/4025154‑4025421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑