Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,052,334	A→G	I190V (ATT→GTT)	typA →	ribosome‑dependent GTPase TypA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,052,334	0	A	G	100.0%	161.4 / NA	48	I190V (ATT→GTT)	typA	ribosome‑dependent GTPase TypA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

TGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCGAAC  >  NZ_CP009273/4052206‑4052468                                                                                                                                 |                                                                                                                                       tGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCa                                                                                                                              >  2:22832/1‑139 (MQ=255)  gTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCAc                                                                                                                             >  2:278227/1‑139 (MQ=255)           ccTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTgcg                                                                                                                    >  1:46025/1‑139 (MQ=255)                            agcagcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGa                                                                                                   <  2:370874/139‑1 (MQ=255)                              cagcTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACg                                                                                                 <  1:568019/139‑1 (MQ=255)                                 cTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTc                                                                                              <  2:185627/139‑1 (MQ=255)                                           cGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCa                                                                                                                              <  2:400118/97‑1 (MQ=255)                                           cGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCa                                                                                                                              >  1:400118/1‑97 (MQ=255)                                                     cGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTtct                                                                          <  1:278227/139‑1 (MQ=255)                                                          cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAg                                                                       <  2:76407/137‑1 (MQ=255)                                                          cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      <  2:531193/138‑1 (MQ=255)                                                          cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      <  2:12162/138‑1 (MQ=255)                                                          cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      <  2:46025/138‑1 (MQ=255)                                                          cTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      <  1:312037/138‑1 (MQ=255)                                                                  aaCGGTATCGCGGGTCTGGGCCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              >  1:395610/1‑138 (MQ=255)                                                                  aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              >  1:152134/1‑138 (MQ=255)                                                                  aaCGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              <  1:322983/138‑1 (MQ=255)                                                                            cgGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              <  2:271612/128‑1 (MQ=255)                                                                            cgGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              >  1:271612/1‑128 (MQ=255)                                                                                tCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGc                                               >  1:306565/1‑139 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACTTTCCTGCGCCGGACGTTGACCTTGa                                                                                                   <  2:86151/78‑1 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTgcgc                                                                                                                   >  1:189472/1‑62 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTgcgc                                                                                                                   <  2:189472/62‑1 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGa                                                                                                   >  1:86151/1‑78 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCCGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:333407/1‑138 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      >  1:571068/1‑107 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGc                                                                      <  2:571068/107‑1 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:313272/1‑138 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  2:365798/1‑138 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:318693/1‑138 (MQ=255)                                                                                         cGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCgg                                       >  1:462702/1‑138 (MQ=255)                                                                                          gAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCa                                     <  2:229355/139‑1 (MQ=255)                                                                                          gAAGAGATGGCGGAAGACATGTCCCCGCTGTACGAGGCGGTGGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTGCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCa                                     <  2:150590/139‑1 (MQ=255)                                                                                              atatGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              >  2:40810/1‑110 (MQ=255)                                                                                              atatGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTac                                                              <  1:40810/110‑1 (MQ=255)                                                                                               tatGGCGGAAGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGc                                <  1:142795/139‑1 (MQ=255)                                                                                                        aGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCt                                                                     <  1:493556/93‑1 (MQ=255)                                                                                                        aGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAg                       >  2:32728/1‑139 (MQ=255)                                                                                                        aGACATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGAAGGTCCGTTCCAGATGCAGATTTCTCAGCt                                                                     >  2:493556/1‑93 (MQ=255)                                                                                                          aCATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAgcg                     <  2:306565/139‑1 (MQ=255)                                                                                                          aCATGACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAgcg                     <  2:152134/139‑1 (MQ=255)                                                                                                              gACCCCGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCgg                  >  2:564061/1‑138 (MQ=255)                                                                                                                   cGCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTAAAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGt            >  1:220881/1‑139 (MQ=255)                                                                                                                    gCTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGt            >  1:116353/1‑138 (MQ=255)                                                                                                                     cTGTACCAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTg           >  2:396291/1‑138 (MQ=255)                                                                                                                          ccAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGcc      >  1:46424/1‑138 (MQ=255)                                                                                                                           cAGGCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCg     >  2:153007/1‑138 (MQ=255)                                                                                                                             ggCGGTTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCGAAc  >  2:556604/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       TGTTCGTTAACCTCGACGCGACCGACGAGCAGCTGGACTTCCCGATCGTTTACGCTTCTGCGCTGAACGGTATCGCGGGTCTGGACCACGAAGATATGGCGGAAGACATGACCCCGCTGTACCAGGCGATTGTTGACCACGTTCCTGCGCCGGACGTTGACCTTGACGGTCCGTTCCAGATGCAGATTTCTCAGCTCGATTACAACAGCTATGTTGGCGTTATCGGCATTGGCCGCATCAAGCGCGGTAAAGTGAAGCCGAAC  >  NZ_CP009273/4052206‑4052468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑