Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,114,961	T→C	E593G (GAA→GGA)	priA ←	primosomal protein N'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,114,961	0	T	C	100.0%	89.8 / NA	27	E593G (GAA→GGA)	priA	primosomal protein N'
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

ATGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCA  >  NZ_CP009273/4114831‑4115087                                                                                                                                   |                                                                                                                               aTGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGt                                                                                                                        >  2:247760/1‑139 (MQ=255)   ggCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGtt                                                                                                                       >  1:157001/1‑138 (MQ=255)       ggTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGTAGCACCACTCCGCCCTGTTTAccc                                                                                                                  >  1:55721/1‑139 (MQ=255)        gTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCg                                                                                                                 >  1:334220/1‑139 (MQ=255)             cggcggTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGc                                                                                                            >  2:198623/1‑139 (MQ=255)               gcggTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGccc                                                                                                          >  1:86768/1‑139 (MQ=255)                          atcatTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCgg                                                                                               <  2:356428/139‑1 (MQ=255)                              ttCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGAc                                                                                           <  1:220605/139‑1 (MQ=255)                                 gccgcTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACACCCGGCACGACCGGCGACCt                                                                                         <  2:157001/138‑1 (MQ=255)                                                              gCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGcc                                                            >  1:443431/1‑138 (MQ=255)                                                                                 gCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCCCGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGaaaa                                        >  2:59447/1‑139 (MQ=255)                                                                                     cGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgc                                    >  2:448598/1‑139 (MQ=255)                                                                                     cGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgc                                    >  2:415561/1‑139 (MQ=255)                                                                                          gCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgcgcgc                                <  2:525042/138‑1 (MQ=255)                                                                                          gCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgcgcgc                                <  2:189039/138‑1 (MQ=255)                                                                                          gCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgcgcgc                                <  2:86768/138‑1 (MQ=255)                                                                                            aaCAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCcgt                             <  2:443431/139‑1 (MQ=255)                                                                                                   gATGTTCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCcgtccacgtc                      <  2:334220/139‑1 (MQ=255)                                                                                                        tCCGGATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGt                  >  1:184274/1‑138 (MQ=255)                                                                                                           ggATGGTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATg              >  1:71308/1‑139 (MQ=255)                                                                                                                gTGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAAc          <  2:184274/138‑1 (MQ=255)                                                                                                                 tGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGCGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAAc          >  2:293420/1‑137 (MQ=255)                                                                                                                 tGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgcgcg                                 >  1:138139/1‑114 (MQ=255)                                                                                                                 tGCGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAgcgcg                                 <  2:138139/114‑1 (MQ=255)                                                                                                                   cGTTTGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGc      >  1:212959/1‑139 (MQ=255)                                                                                                                       tGCAGCACCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTc   <  2:90373/138‑1 (MQ=255)                                                                                                                        gcagcaCCACTCCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTca  <  1:324741/138‑1 (MQ=255)                                                                                                                                   |                                                                                                                               ATGGCTGGTCCACGGCGGTAGCTGCATCATTCGCCGCTCAGCCAGCGCCTGTTCGGCAAAGGCGTCGTAGCCTTTATAGAGCAACGTTTGCAACAGAGGATGTTCCGGATGGTGCGTTTGCAGCACCACTTCGCCCTGTTTACCCGCACGCCCGGCACGACCGGCGACCTGGGTGTAAAGCTGAGCGAAACGCTCTGCCGAGCGAAAATCGGCAGAAAACAGCGCGCCGTCCACGTCCAGTAATGCAACCAGCGTCA  >  NZ_CP009273/4114831‑4115087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑