Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,132,297	T→C	intergenic (‑148/‑161)	ptsP ← / → frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,132,297	0	T	C	100.0%	108.3 / NA	31	intergenic (‑148/‑161)	ptsP/frwC	phosphoenolpyruvate‑‑protein phosphotransferase/PTS fructose transporter subunit EIIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

GTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATA  >  NZ_CP009273/4132168‑4132423                                                                                                                                  |                                                                                                                               gTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCCGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTAt                                                                                                                       <  1:232274/139‑1 (MQ=255)     tCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGc                                                                                                                    <  1:225179/138‑1 (MQ=255)          ttACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGtt                                                                                                               <  2:150478/138‑1 (MQ=255)            aCAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTAt                                                                                                             >  1:191594/1‑138 (MQ=255)                        cgcACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCa                                                                                                >  1:404221/1‑139 (MQ=255)                                       tGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCg                                                                                                  >  2:351076/1‑122 (MQ=255)                                       tGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCg                                                                                                  <  1:351076/122‑1 (MQ=255)                                       tGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGatat                                                                                 >  1:495243/1‑139 (MQ=255)                                         ccATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGa                                                                                                     >  1:34088/1‑117 (MQ=255)                                         ccATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGa                                                                                                     <  2:34088/117‑1 (MQ=255)                                         ccATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTa                                                                               >  2:10600/1‑139 (MQ=255)                                           aTGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACa                                                                             <  1:165917/139‑1 (MQ=255)                                            tgtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                            <  1:255429/139‑1 (MQ=255)                                            tgtgACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACaa                                                                            <  2:110437/139‑1 (MQ=255)                                                               gCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCtt                                                         <  2:282088/139‑1 (MQ=255)                                                                   gACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGcc                                                           <  1:357834/133‑1 (MQ=255)                                                                   gACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGcc                                                           >  2:357834/1‑133 (MQ=255)                                                                      aaaTGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATcc                                                  <  1:150581/139‑1 (MQ=255)                                                                             atgtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGttt                                           >  1:498075/1‑139 (MQ=255)                                                                               gtaaCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGc                                         >  2:546390/1‑139 (MQ=255)                                                                                  aCCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTcc                                      >  2:242873/1‑139 (MQ=255)                                                                                   ccGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGc                                                                                                                    <  1:395115/60‑1 (MQ=255)                                                                                   ccGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGc                                                                                                                    >  2:395115/1‑60 (MQ=255)                                                                                   ccGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCg                                     >  1:450392/1‑139 (MQ=255)                                                                                    cGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGc                                    <  2:191594/139‑1 (MQ=255)                                                                                                aCGCGTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCt                        <  1:546390/139‑1 (MQ=255)                                                                                                    gTCTCACAAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTg                    >  1:349320/1‑139 (MQ=255)                                                                                                         acaAGACGCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGtt                  <  2:498075/136‑1 (MQ=255)                                                                                                                gCTGTTTTGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATc         >  2:583159/1‑138 (MQ=255)                                                                                                                       tGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAata  <  1:409895/138‑1 (MQ=255)                                                                                                                       tGCGGCATGCTCCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAata  <  1:10600/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                               GTTTTCTGTTTACAGGATAAGAGCGCACGGCAACGGCCTGCCATGTGACAAATCTGCCAAAAGCTGGACAAATGTAATGTAACCGTCAATTTGCGACGCGTCTCACAAGACGCTGTTTTGCGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATA  >  NZ_CP009273/4132168‑4132423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑