Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,172,602	A→C	E477A (GAG→GCG)	rpoB →	DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,172,602	0	A	C	100.0%	96.4 / NA	29	E477A (GAG→GCG)	rpoB	DNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/17);  total (12/17)

ATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT  >  NZ_CP009273/4172469‑4172736                                                                                                                                      |                                                                                                                                       aTCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCgt                                                                                                                                    <  1:191305/138‑1 (MQ=255)          cGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTgg                                                                                                                          <  2:225189/139‑1 (MQ=255)              aCGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGa                                                                                                                      <  2:575599/139‑1 (MQ=255)              aCGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGa                                                                                                                      <  1:433929/139‑1 (MQ=255)                            gTCGATGATATCGACCACCTCGGCAGCCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                                                                                                        <  1:281481/139‑1 (MQ=255)                                 tgatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca                                                                                                   <  2:277685/139‑1 (MQ=255)                                 tgatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca                                                                                                   <  2:181836/139‑1 (MQ=255)                                   atatCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcac                                                                                                  <  1:475028/138‑1 (MQ=255)                                   atatCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcac                                                                                                  <  1:558715/138‑1 (MQ=255)                                             ccTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca                                                                                                   >  2:36102/1‑127 (MQ=255)                                             ccTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca                                                                                                   <  1:36102/127‑1 (MQ=255)                                                                 gttccgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgc                                                                   <  1:109263/139‑1 (MQ=255)                                                                 gttccgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCgc                                                                   <  1:78401/139‑1 (MQ=255)                                                                      gttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcac                                                                                                  >  2:99733/1‑103 (MQ=255)                                                                      gttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcac                                                                                                  <  1:99733/103‑1 (MQ=255)                                                                                   cGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGttctt                                                 <  1:322321/139‑1 (MQ=255)                                                                                       aaaCCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGt                                             <  1:319459/139‑1 (MQ=255)                                                                                              ttCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTccagc                                       >  2:287051/1‑138 (MQ=255)                                                                                                                  tgtAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGAc                  >  1:567930/1‑139 (MQ=255)                                                                                                                  tgtAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGAc                  >  2:206145/1‑139 (MQ=255)                                                                                                                  tgtAGAGCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGAc                  >  1:315976/1‑139 (MQ=255)                                                                                                                       agCGTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGaa             >  1:124382/1‑139 (MQ=255)                                                                                                                          gTGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGaacaa          >  1:25604/1‑139 (MQ=255)                                                                                                                           tGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGaacaac         >  1:554359/1‑139 (MQ=255)                                                                                                                           tGCGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGaacaac         >  2:191808/1‑139 (MQ=255)                                                                                                                             cGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGAc                  >  1:324086/1‑128 (MQ=255)                                                                                                                             cGGTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGAc                  <  2:324086/128‑1 (MQ=255)                                                                                                                              ggTGAAAGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCg      <  1:206145/139‑1 (MQ=255)                                                                                                                                  aaaGCGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGt  >  2:64176/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                       ATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGT  >  NZ_CP009273/4172469‑4172736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑