Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,235,276	T→C	N358D (AAC→GAC)	malE ←	maltose/maltodextrin ABC transporter substrate‑binding protein MalE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,235,276	0	T	C	100.0%	149.2 / NA	43	N358D (AAC→GAC)	malE	maltose/maltodextrin ABC transporter substrate‑binding protein MalE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

CGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAG  >  NZ_CP009273/4235142‑4235402                                                                                                                                       |                                                                                                                               cGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGc                                                                                                                            <  1:90663/139‑1 (MQ=255)     aTTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGa                                                                                                                        <  2:13206/139‑1 (MQ=255)                   cTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTgg                                                                                                          <  2:113361/139‑1 (MQ=255)                      ggTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCg                                                                                                       >  1:64660/1‑139 (MQ=255)                       gTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCg                                                                                                       <  2:359554/138‑1 (MQ=255)                           tACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTc                                                                                                  >  1:291818/1‑139 (MQ=255)                                 tCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCAtggt                                                                                            >  1:160397/1‑139 (MQ=255)                                  cTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCAtggtg                                                                                           >  1:394362/1‑139 (MQ=255)                                     cgcgTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTggcg                                                                                        >  1:557308/1‑139 (MQ=255)                                       cgTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTggcggc                                                                                      <  2:291818/139‑1 (MQ=255)                                         tCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCaa                                                                                    >  1:422396/1‑139 (MQ=255)                                          cTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCaa                                                                                    >  2:256213/1‑138 (MQ=255)                                                                          cTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                                      >  2:20260/1‑136 (MQ=255)                                                                          cTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                                      >  1:47821/1‑136 (MQ=255)                                                                              cggcgTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACtt                                               <  1:404756/139‑1 (MQ=255)                                                                                     gATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTg                                                                              >  2:229142/1‑101 (MQ=255)                                                                                     gATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTg                                                                              <  1:229142/101‑1 (MQ=255)                                                                                           cGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCa                                  >  2:314988/1‑139 (MQ=255)                                                                                           cGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCa                                  >  1:90166/1‑139 (MQ=255)                                                                                                     aCGGCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGtt                        <  2:301600/139‑1 (MQ=255)                                                                                                       ggCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTg                      <  2:557308/139‑1 (MQ=255)                                                                                                        gCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt                     <  1:95627/139‑1 (MQ=255)                                                                                                        gCATACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGt                     <  1:143908/139‑1 (MQ=255)                                                                                                          aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                                      <  1:107553/104‑1 (MQ=255)                                                                                                          aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGt                                                      >  2:107553/1‑104 (MQ=255)                                                                                                          aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCt                   >  2:271479/1‑139 (MQ=255)                                                                                                          aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCt                   >  1:170071/1‑139 (MQ=255)                                                                                                          aTACCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCt                   >  1:427817/1‑139 (MQ=255)                                                                                                            aCCAGAAAGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCtt                  >  1:7593/1‑138 (MQ=255)                                                                                                                  aaGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAc           <  1:39139/139‑1 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTggcggc                                                                                      <  2:24507/63‑1 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTggcggc                                                                                      >  1:24507/1‑63 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc          <  1:314988/139‑1 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc          >  2:284657/1‑139 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc          >  1:509096/1‑139 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc          <  2:509096/139‑1 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc          >  1:63262/1‑139 (MQ=255)                                                                                                                   aGCGGACATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGCGGCGGCAATCCGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAAcc          >  2:130376/1‑139 (MQ=255)                                                                                                                         cATCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTcc    <  2:510248/139‑1 (MQ=255)                                                                                                                          aTCTGCGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCa   <  1:378396/139‑1 (MQ=255)                                                                                                                               cGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAg  <  2:288011/135‑1 (MQ=255)                                                                                                                               cGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAg  <  1:130376/135‑1 (MQ=255)                                                                                                                               cGGGATGTCCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAg  <  2:47821/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                               CGGCATTTCACAGCATTACTTGGTGATACGAGTCTGCGCGTCTTTCAGGGCTTCATCGACAGTCTGACGACCGCTGGCGGCGTTGATCACCGCAGTACGCACGGCATACCAGAAAGCGGACATCTGCGGGATGTTCGGCATGATTTCACCTTTCTGGGCGTTTTCCATGGTGGCGGCAATACGTGGATCTTTCGCCAACTCTTCCTCGTAAGACTTCAGCGCTACGGCACCCAGCGGTTTGTCTTTATTAACCGCTTCCAG  >  NZ_CP009273/4235142‑4235402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑