Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,262,930	A→T	D290E (GAT→GAA)	uvrA ←	excinuclease ABC subunit UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,262,930	0	A	T	100.0%	79.3 / NA	21	D290E (GAT→GAA)	uvrA	excinuclease ABC subunit UvrA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/13);  total (8/13)

CTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGC  >  NZ_CP009273/4262800‑4263065                                                                                                                                   |                                                                                                                                        cTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAata                                                                                                                                 >  1:389643/1‑139 (MQ=255)    cGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAatat                                                                                                                                <  1:192752/137‑1 (MQ=255)    cGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAatat                                                                                                                                <  1:207172/137‑1 (MQ=255)       cGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTg                                                                                                                           <  2:462369/139‑1 (MQ=255)       cGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTg                                                                                                                           <  1:23541/139‑1 (MQ=255)            aaCTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGc                                                                                                                      >  1:67305/1‑139 (MQ=255)                       aTCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCg                                                                                                           >  2:133088/1‑139 (MQ=255)                         cTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTg                                                                                                                           >  1:234095/1‑121 (MQ=255)                         cTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTg                                                                                                                           <  2:234095/121‑1 (MQ=255)                                     tCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAgg                                                                                              <  2:300878/138‑1 (MQ=255)                                       aGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGcc                                                                                            <  2:347955/138‑1 (MQ=255)                                                  ataataGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGgttgt                                                                               <  2:389643/139‑1 (MQ=255)                                                              gcggcgATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGaaaa                                                                     >  1:528834/1‑137 (MQ=255)                                                                        cAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTc                                                         <  1:365413/139‑1 (MQ=255)                                                                        cAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTc                                                         <  1:133088/139‑1 (MQ=255)                                                                                     caccacCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                              <  2:179526/137‑1 (MQ=255)                                                                                     caccacCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCa                                              >  1:179526/1‑137 (MQ=255)                                                                                           cagccagcGACAGTTCCGGCTTCTGGATCACTCGATCAGGTGCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGACAAACAGG‑CCGCGCCGGGCGGTTAAACGAAAACAGTCGCGGTTCCAGGTCACGCCGACTGTAg                                      >  2:263800/1‑139 (MQ=255)                                                                                                agcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt                                                          <  1:401808/114‑1 (MQ=255)                                                                                                agcGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt                                                          >  2:401808/1‑114 (MQ=255)                                                                                                                                aGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAAcagc  <  2:185228/138‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CTTCGACGTCGAACTTATAGTGATCTGCCAGCGATTTCAGCATCTGGAAATAATAGAAGTTGCGGCGATCCCAGCCACGGATCGCACCACCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGGAGAACAGC  >  NZ_CP009273/4262800‑4263065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑