Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,264,031	A→T	intergenic (‑232/‑22)	uvrA ← / → ssb1	excinuclease ABC subunit UvrA/single‑stranded DNA‑binding protein SSB1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,264,031	0	A	T	100.0%	172.4 / NA	42	intergenic (‑232/‑22)	uvrA/ssb1	excinuclease ABC subunit UvrA/single‑stranded DNA‑binding protein SSB1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/17);  total (25/17)

ATTGGAATGCATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCC  >  NZ_CP009273/4263902‑4264166                                                                                                                                  |                                                                                                                                        aTTGGAATGCATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGtttttttt                                                                                                                                  >  2:194463/1‑137 (MQ=255)  ttGGAATGCATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAg                                                                                                                               >  1:95718/1‑139 (MQ=255)       aTGCATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGac                                                                                                                          <  2:211068/139‑1 (MQ=255)        tGCATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGaca                                                                                                                         >  1:195544/1‑139 (MQ=255)          cATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGacac                                                                                                                        >  2:451504/1‑138 (MQ=255)                    aGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAAc                                                                                                                    <  2:267477/132‑1 (MQ=255)                    aGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAAc                                                                                                                    >  1:267477/1‑132 (MQ=255)                    aGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGc                                                                                                               >  1:103031/1‑137 (MQ=255)                    aGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGc                                                                                                               >  1:82810/1‑137 (MQ=255)                               aCAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTa                                                                                                  <  2:195544/139‑1 (MQ=255)                                cAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTa                                                                                                  >  1:576960/1‑138 (MQ=255)                                   tGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATg                                                                                                                 >  2:386284/1‑120 (MQ=255)                                   tGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATg                                                                                                                 <  1:386284/120‑1 (MQ=255)                                     tCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAg                                                                                            >  2:27963/1‑139 (MQ=255)                                     tCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAg                                                                                            >  1:173788/1‑139 (MQ=255)                                             ggTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATtct                                                                                    <  1:194463/139‑1 (MQ=255)                                              gtttgtttCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATtctc                                                                                   >  2:328084/1‑139 (MQ=255)                                                        cGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATc                                                                         >  1:431464/1‑139 (MQ=255)                                                             ccGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGt                                                                    <  2:95718/139‑1 (MQ=255)                                                                             gTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTa                                                     <  2:87787/138‑1 (MQ=255)                                                                                 aaGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTa                                                >  2:53219/1‑139 (MQ=255)                                                                                   gcgcTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACa                                              >  1:475578/1‑139 (MQ=255)                                                                                                 ggTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTaaa                                                                                                <  1:307848/75‑1 (MQ=255)                                                                                                 ggTACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTaaa                                                                                                >  2:307848/1‑75 (MQ=255)                                                                                                   tACAATCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGgcg                               >  2:285028/1‑138 (MQ=255)                                                                                                      aaTCGCGCGTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGt                           >  2:510976/1‑139 (MQ=255)                                                                                                           cgcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAgg                                                                                                      <  2:488335/59‑1 (MQ=255)                                                                                                           cgcgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAgg                                                                                                      >  1:488335/1‑59 (MQ=255)                                                                                                             cgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGc                        >  2:113142/1‑135 (MQ=255)                                                                                                             cgTTTACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGc                        <  1:113142/135‑1 (MQ=255)                                                                                                               tttACACTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACAt                  <  1:285028/139‑1 (MQ=255)                                                                                                                  acacTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCCGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCt                                                 >  2:529709/1‑105 (MQ=255)                                                                                                                  acacTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCt                                                 <  1:529709/105‑1 (MQ=255)                                                                                                                   cacTTATTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACg              <  2:103031/139‑1 (MQ=255)                                                                                                                        aTTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTa                                                     <  2:515954/95‑1 (MQ=255)                                                                                                                        aTTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTa                                                     >  1:515954/1‑95 (MQ=255)                                                                                                                        aTTCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGc         >  1:469878/1‑139 (MQ=255)                                                                                                                          tCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTAc                                               >  1:99041/1‑99 (MQ=255)                                                                                                                          tCAGAACGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTAc                                               <  2:99041/99‑1 (MQ=255)                                                                                                                              aaCGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTa                                                     <  2:336611/89‑1 (MQ=255)                                                                                                                              aaCGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTa                                                     >  1:336611/1‑89 (MQ=255)                                                                                                                              aaCGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTc   >  2:302591/1‑139 (MQ=255)                                                                                                                               aCGTTTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTcc  <  1:451504/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        ATTGGAATGCATTACCCGGAGTGTTGTGTAACAATGTCTGGCCAGGTTTGTTTCCCGGAACCGAGGTCACAACATAGTAAAAGCGCTATTGGTAATGGTACAATCGCGCGTTTACACTTATTCAGAACGATTTTTTTCAGGAGACACGAACATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCC  >  NZ_CP009273/4263902‑4264166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑