Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,377,323	T→C	D622G (GAC→GGC)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,377,323	0	T	C	100.0%	126.3 / NA	37	D622G (GAC→GGC)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

GTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGTCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGG  >  NZ_CP009273/4377188‑4377457                                                                                                                                        |                                                                                                                                       gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGgccgt                                                                                                                                     >  2:146064/1‑139 (MQ=255)          tCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCACAGGGTGACCACCGCCAGCGCACCGCCCATGAGGATAAAGCAACGCCGTCCCAGCGAACCGGAGACTTCACGGCCACCGAGGTTa                                                                                                                             >  2:506324/1‑138 (MQ=255)               gcTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGaa                                                                                                                        >  2:424446/1‑138 (MQ=255)                          tgttgAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAgcg                                                                                                             <  2:341388/138‑1 (MQ=255)                                gaTACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAAt                                                                                                      <  1:146064/139‑1 (MQ=255)                                  tACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGACCGTCGAGGTTATCGAACATCATCAGCGCCATAAt                                                                                                      >  1:269015/1‑137 (MQ=255)                                    cAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAg                                                                                                   <  2:47542/138‑1 (MQ=255)                                    cAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGc                                                                                                  >  1:47542/1‑139 (MQ=255)                                    cAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGCGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGc                                                                                                  >  1:498224/1‑139 (MQ=255)                                           aTCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAGGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACaa                                                                                           >  1:581991/1‑139 (MQ=255)                                           aTCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACaa                                                                                           >  1:8372/1‑139 (MQ=255)                                            tCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACaa                                                                                           >  2:522413/1‑138 (MQ=255)                                               cGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCgg                                                                                                >  2:308458/1‑130 (MQ=255)                                               cGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCgg                                                                                                <  1:308458/130‑1 (MQ=255)                                                 gCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGcc                                                                                     >  1:419343/1‑139 (MQ=255)                                                       ttCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATg                                                                                <  1:341989/138‑1 (MQ=255)                                                          aGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtca                                                                            <  1:401143/139‑1 (MQ=255)                                                          aGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtca                                                                            <  1:451055/139‑1 (MQ=255)                                                           ggCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtcat                                                                           <  1:552298/139‑1 (MQ=255)                                                            gCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGAt                                                                                 >  2:502357/1‑132 (MQ=255)                                                            gCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGAt                                                                                 <  1:502357/132‑1 (MQ=255)                                                                aGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAgg                                                                       <  2:488790/138‑1 (MQ=255)                                                                         accGCCAGCGCACCGCAAATGAGGATAAGGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCa                                                             <  2:581991/139‑1 (MQ=255)                                                                          ccGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCgg                                                                                                >  2:242946/1‑103 (MQ=255)                                                                          ccGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCgg                                                                                                <  1:242946/103‑1 (MQ=255)                                                                                gcgcACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACg                                                      >  1:488093/1‑139 (MQ=255)                                                                                gcgcACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACg                                                      <  2:488093/139‑1 (MQ=255)                                                                                           aTGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAgcg                                                               <  2:296154/119‑1 (MQ=255)                                                                                           aTGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAgcg                                                               >  1:296154/1‑119 (MQ=255)                                                                                                          agcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGgccagcca                             >  1:19979/1‑138 (MQ=255)                                                                                                          agcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGgccagcca                             >  1:430320/1‑138 (MQ=255)                                                                                                           gcCGTCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCaaa                           <  2:525272/139‑1 (MQ=255)                                                                                                               tCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtcatc                                                                          <  2:400379/88‑1 (MQ=255)                                                                                                               tCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCtcatc                                                                          >  1:400379/1‑88 (MQ=255)                                                                                                               tCCCAGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAAt                         >  1:46573/1‑137 (MQ=255)                                                                                                                   aGCGAACCGGAGAATTCACGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGa                   <  2:430320/139‑1 (MQ=255)                                                                                                                                    aCGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTcggg  <  2:8372/139‑1 (MQ=255)                                                                                                                                    aCGGCCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGGAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTcggg  <  1:506324/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAGCAAAGCCGTCCCAGCGAACCGGAGAATTCACGGTCGTCGAGGTTATCGAACATCATCAGCGCCATAATCAGCGGCACAATAAGCCCGATGCTCATCAGGTAGTAGCGCATCCCACGGGAAACACGTTCGCGCGGCCAGCCAAAATGAGCGATAAACAAGCCGTTCGGG  >  NZ_CP009273/4377188‑4377457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑