Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,409,650	T→C	intergenic (‑208/‑147)	ulaG ← / → ulaA	L‑ascorbate 6‑phosphate lactonase/PTS ascorbate transporter subunit IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,409,650	0	T	C	100.0%	57.2 / NA	17	intergenic (‑208/‑147)	ulaG/ulaA	L‑ascorbate 6‑phosphate lactonase/PTS ascorbate transporter subunit IIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

TTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAATCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTACCTC  >  NZ_CP009273/4409515‑4409779                                                                                                                                        |                                                                                                                                  ttCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAAccc                                                                                                                                 <  2:409624/138‑1 (MQ=255)          gCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATcc                                                                                                                       <  1:416778/139‑1 (MQ=255)                                      gggAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTca                                                                                            >  2:43886/1‑138 (MQ=255)                                         aaGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACAtt                                                                                        <  2:157022/139‑1 (MQ=255)                                              gttgttgATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAAt                                                                                    <  2:360563/138‑1 (MQ=255)                                                 gttgATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCat                                                                                 >  2:196566/1‑138 (MQ=255)                                                    gATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCataaa                                                                              <  2:247526/138‑1 (MQ=255)                                                                           aTGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGa                                                      <  1:370020/139‑1 (MQ=255)                                                                                 tgtgTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAAt                                                  >  2:24583/1‑137 (MQ=255)                                                                                             ttAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGcac                                    <  1:241145/139‑1 (MQ=255)                                                                                             ttAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGca                                     >  2:452171/1‑138 (MQ=255)                                                                                                aCTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCacaa                                  <  1:43886/138‑1 (MQ=255)                                                                                                     aTGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATcc                                                                                                                       >  2:295360/1‑48 (MQ=255)                                                                                                     aTGAATTTAAATGGATAATTGTTTCGTTGTGTGAACCCCACTCTATcc                                                                                                                       <  1:295360/48‑1 (MQ=255)                                                                                                                            tCGTTGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTAc     >  1:21936/1‑139 (MQ=255)                                                                                                                               ttGTGTGAACCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTACctc  >  1:384386/1‑139 (MQ=255)                                                                                                                                  tgtgAACCCCACTCTATCCATGTGGAATTATTTGCggg                                                                                                    <  1:461226/38‑1 (MQ=255)                                                                                                                                  tgtgAACCCCACTCTATCCATGTGGAATTATTTGCggg                                                                                                    >  2:461226/1‑38 (MQ=255)                                                                                                                                        |                                                                                                                                  TTCAGGAAGGCAATGACCATTTTTTGACTTTTTGCCAGGGAAGTTGTTGTTGATTTTGAGTATGGAAAGATTTAATGGAATGTGTAATTCAATTAACTAAATGAATTTAAATGGATAATTGTTTCGTTGTGTGAATCCCACTCTATCCATGTGGAATTATTTGCGGGTCGCGTCACATTTAATCATAAATAATCTTGTTGTGATTACTTTTGAAAATTAGAGTGAGTGCACAACATTCCGGGTGTGTGGAATACCCGGTTACCTC  >  NZ_CP009273/4409515‑4409779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑