Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,428,964	T→C	E39G (GAG→GGG)	ytfJ ←	YtfJ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,428,964	0	T	C	100.0%	120.0 / NA	35	E39G (GAG→GGG)	ytfJ	YtfJ family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

TCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGATA  >  NZ_CP009273/4428830‑4429099                                                                                                                                       |                                                                                                                                        tCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGcc                                                                                                                                     <  2:253576/139‑1 (MQ=255)       cAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCACCCCGCCCCGATc                                                                                                                               >  2:270607/1‑139 (MQ=255)                                       gTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCgg                                                                                               >  1:170861/1‑139 (MQ=255)                                          cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGttt                                                                                            >  2:243664/1‑139 (MQ=255)                                          cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGtt                                                                                             <  2:341729/138‑1 (MQ=255)                                                   atatGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAt                                                                                   <  1:243664/139‑1 (MQ=255)                                                      tGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAtgtg                                                                                <  1:566161/139‑1 (MQ=255)                                                           cAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCgg                                                                             >  1:133795/1‑137 (MQ=255)                                                                   gCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  <  1:26469/108‑1 (MQ=255)                                                                   gCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  >  2:26469/1‑108 (MQ=255)                                                                        tttCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGc                                                               >  2:279636/1‑138 (MQ=255)                                                                                   cTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCggcggc                                                                                                            >  2:370498/1‑82 (MQ=255)                                                                                   cTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCggcggc                                                                                                            <  1:370498/82‑1 (MQ=255)                                                                                   cTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAgcaggcag                                                   >  2:490626/1‑139 (MQ=255)                                                                                      cgcgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTg                                                <  2:133795/139‑1 (MQ=255)                                                                                        cgcTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAg                                              >  2:7336/1‑139 (MQ=255)                                                                                             ttCCAGGTTTTGTAGCTAACCTGATTTTTATCAAGCACCACCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCa                                         <  1:267553/139‑1 (MQ=255)                                                                                             ttCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCa                                         <  2:285219/139‑1 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCg                                                                                                                                  <  1:291378/40‑1 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCg                                                                                                                                  >  2:291378/1‑40 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAtgtg                                                                                >  1:451771/1‑90 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAtgtg                                                                                <  2:451771/90‑1 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTg                                 <  1:521688/137‑1 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTg                                 >  2:521688/1‑137 (MQ=255)                                                                                                       tGTAGCTAAACTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               >  1:416656/1‑139 (MQ=255)                                                                                                               aaCTGATCTTTATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCa                       <  1:270607/139‑1 (MQ=255)                                                                                                                         tATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  <  2:76609/54‑1 (MQ=255)                                                                                                                         tATCAAGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                                                                                  >  1:76609/1‑54 (MQ=255)                                                                                                                              aGCACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCcttct         >  1:532031/1‑138 (MQ=255)                                                                                                                                cACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTAt      >  1:26563/1‑139 (MQ=255)                                                                                                                                cACCAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTAt      >  2:526926/1‑139 (MQ=255)                                                                                                                                   cAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               <  2:288633/111‑1 (MQ=255)                                                                                                                                   cAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCg                               >  1:288633/1‑111 (MQ=255)                                                                                                                                   cAACCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGa    >  2:543608/1‑138 (MQ=255)                                                                                                                                    aaCCCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGata  <  1:265404/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCATCGGCAACAGCAGGCAGGTGAGTGCCAGAATCTTGCGTAGGGTCATGGTGTTTCCTTCTTATGATA  >  NZ_CP009273/4428830‑4429099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑