Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,493,312	A→G	intergenic (‑46/‑563)	insG ← / → nanX	IS4‑like element IS4 family transposase/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,493,312	0	A	G	100.0%	119.4 / NA	33	intergenic (‑46/‑563)	insG/nanX	IS4‑like element IS4 family transposase/MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/15);  total (18/15)

AGTCAGTGGGTTACGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGG  >  NZ_CP009273/4493186‑4493442                                                                                                                               |                                                                                                                                   aGTCAGTGGGTTACGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGAt                                                                                                                        <  2:110207/139‑1 (MQ=255)  gTCAGTGGGTTACGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATc                                                                                                                       >  2:70719/1‑139 (MQ=255)   tCAGTGGGTTACGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCg                                                                                                                      >  2:502296/1‑139 (MQ=255)   tCAGTGGGTTACGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCg                                                                                                                      >  2:72009/1‑139 (MQ=255)             aCGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCt                                                                                                            >  2:340532/1‑139 (MQ=255)              cacAGAGAATCGTAACGGGATGCCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCt                                                                                                            <  1:229793/136‑1 (MQ=255)              cGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCt                                                                                                            <  1:429736/138‑1 (MQ=255)              cGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCt                                                                                                            <  2:11002/138‑1 (MQ=255)                                               gCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGAt                                                                                                                        >  1:204764/1‑93 (MQ=255)                                               gCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGAt                                                                                                                        <  2:204764/93‑1 (MQ=255)                                               gCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTAc                                                                           >  1:126345/1‑138 (MQ=255)                                                  tGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACt                                                                                    <  2:539680/126‑1 (MQ=255)                                                  tGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACt                                                                                    >  1:539680/1‑126 (MQ=255)                                                  tGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACaaa                                                                        >  2:59839/1‑138 (MQ=255)                                                           tgCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTc                                                               >  2:53711/1‑138 (MQ=255)                                                              aTAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAAtt                                                           >  2:532268/1‑139 (MQ=255)                                                                      aTCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAt                                                   <  2:429767/139‑1 (MQ=255)                                                                                       cGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGtt                                  <  1:53711/139‑1 (MQ=255)                                                                                         tttCCGGATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGtttt                                <  2:126345/139‑1 (MQ=255)                                                                                               gATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAt                                                   >  2:200794/1‑114 (MQ=255)                                                                                               gATTCTTACACAGCCACTGGATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACAt                                                   <  1:200794/114‑1 (MQ=255)                                                                                                    ttACACAGCTACTGGATCGGTCAACTGGTCCTTAAGTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGAt                     >  2:311627/1‑139 (MQ=255)                                                                                                                  gATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGa       <  1:503927/139‑1 (MQ=255)                                                                                                                  gATCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGa       <  2:538358/139‑1 (MQ=255)                                                                                                                    tCGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATc     >  2:120433/1‑139 (MQ=255)                                                                                                                     cGGTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAGCAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCt    >  2:367074/1‑139 (MQ=255)                                                                                                                      ggTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCa                                                 >  2:421745/1‑93 (MQ=255)                                                                                                                      ggTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCa                                                 <  1:421745/93‑1 (MQ=255)                                                                                                                      ggTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTg   <  1:70719/139‑1 (MQ=255)                                                                                                                       gTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGtttt                                <  1:182921/109‑1 (MQ=255)                                                                                                                       gTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGtttt                                >  2:182921/1‑109 (MQ=255)                                                                                                                       gTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTgg  >  1:434726/1‑139 (MQ=255)                                                                                                                       gTCAACTGGTCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTgg  >  2:94780/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                   AGTCAGTGGGTTACGCAGAGAATCGTAACGGGATACCAGATCAAGAGCCTGTCCAATGTGCATAAAAAAATCCGGAAACAAGTGAGCGTTTCCGGATTCTTACACAGCCACTGGATCGGTCAACTGATCCTTAACTGATCGGCATTAATCTTGGTTCTGGTGTTTGTAACAAACTATCAGCTACAAAAATATGCTCAATTTGTGACATCAGTAACAAAACGCGTTTTGTTATGTGGATTGCTTGTTTTTTGATCTGG  >  NZ_CP009273/4493186‑4493442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑