| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,501,175 | T→C | I34V (ATC→GTC) | fecE ← | Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,501,175 | 0 | T | C | 96.3% | 89.2 / ‑5.8 | 27 | I34V (ATC→GTC) | fecE | Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (14/12); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACA > NZ_CP009273/4501065‑4501302 | tGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGt > 2:268982/1‑139 (MQ=255)tGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGt < 1:144689/139‑1 (MQ=255) aTGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcg < 1:165774/138‑1 (MQ=255) tGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcgtc > 1:384370/1‑139 (MQ=255) aTTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTa < 1:484495/139‑1 (MQ=255) ttATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGtt > 1:71147/1‑137 (MQ=255) tATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTa < 2:71147/137‑1 (MQ=255) aTCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg > 2:162875/1‑138 (MQ=255) aTCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg > 1:507734/1‑138 (MQ=255) aTCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAg > 1:563429/1‑138 (MQ=255) gCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTAc < 2:45864/138‑1 (MQ=255) agaAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTtgt > 2:61766/1‑139 (MQ=255) tGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCa < 1:53813/75‑1 (MQ=255) tGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCa > 2:53813/1‑75 (MQ=255) ccAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAATGTCGtt < 2:200489/116‑1 (MQ=255) ccAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAATGTCGtt > 1:200489/1‑116 (MQ=255) ccAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAAc > 2:139377/1‑139 (MQ=255) cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcg < 1:308709/103‑1 (MQ=255) cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAAcg > 2:308709/1‑103 (MQ=255) cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGt < 1:193885/138‑1 (MQ=255) cGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGt > 2:440642/1‑138 (MQ=255) aaaGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTc < 1:268982/139‑1 (MQ=255) gTCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATtc < 1:69641/139‑1 (MQ=255) tCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATtct > 1:254063/1‑139 (MQ=255) tCGATTTCCCGCAACCGTTAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTAGTAAAGTCATTTCTCGCATtct > 2:422083/1‑139 (MQ=255) ccGTTAGAACCGACCATGGCGGTGATCTTCCGCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTGCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGa < 2:328768/139‑1 (MQ=255) ttAGGACCGACCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACa > 1:165332/1‑138 (MQ=255) | TGAGAGCATATTTATGGGATTATCGCCGAGAAATACGGTGCCAGACTGCGGCATTAAAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACCGATCAGGGCGGTGATCTTCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACA > NZ_CP009273/4501065‑4501302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |