Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,531,167 G→A A132T (GCC→ACC)  fimB → type 1 fimbria switch DNA invertase FimB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,531,1670GA97.4% 135.7 / ‑3.6 38A132T (GCC→ACC) fimBtype 1 fimbria switch DNA invertase FimB
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (16/21);  total (17/21)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

CAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGT  >  NZ_CP009273/4531032‑4531299
                                                                                                                                       |                                                                                                                                    
cAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCg                                                                                                                                   >  2:58285/1‑139 (MQ=255)
 aGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCgg                                                                                                                                  >  2:38262/1‑139 (MQ=255)
         aaaaaCTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCa                                                                                                                           <  2:447152/138‑1 (MQ=255)
                             tACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATg                                                                                                      <  1:38262/139‑1 (MQ=255)
                                    tACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGc                                                                                                >  2:239901/1‑138 (MQ=255)
                                       ccGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCAtt                                                                                            >  2:71304/1‑139 (MQ=255)
                                          cATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgt                                                                                         >  2:314450/1‑139 (MQ=255)
                                           aTGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCgtg                                                                                        <  1:42462/139‑1 (MQ=255)
                                                     cGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCt                                                                              <  1:239901/139‑1 (MQ=255)
                                                       aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCttt                                                                            >  2:581094/1‑139 (MQ=255)
                                                       aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCttt                                                                            >  1:315047/1‑139 (MQ=255)
                                                       aGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCttt                                                                            >  2:291709/1‑139 (MQ=255)
                                                           ggTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCg                                                                        <  1:138377/139‑1 (MQ=255)
                                                               tttttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAata                                                                    <  1:71304/139‑1 (MQ=255)
                                                                 tttATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                                   <  1:69547/138‑1 (MQ=255)
                                                                       aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                                   <  1:129875/132‑1 (MQ=255)
                                                                       aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAatat                                                                   >  2:129875/1‑132 (MQ=255)
                                                                       aCGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAAt                                                             <  1:581061/138‑1 (MQ=255)
                                                                                   tCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACt                                                 >  2:456288/1‑138 (MQ=255)
                                                                                   tCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGAt                                                                                                                   <  2:415473/72‑1 (MQ=255)
                                                                                   tCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGAt                                                                                                                   >  1:415473/1‑72 (MQ=255)
                                                                                   tCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACt                                                 >  1:360724/1‑138 (MQ=255)
                                                                                              ggCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                                        <  1:60965/136‑1 (MQ=255)
                                                                                              ggCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                                        <  1:581094/136‑1 (MQ=255)
                                                                                              ggCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGa                                        <  1:291709/136‑1 (MQ=255)
                                                                                                     gTTTTACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg                              <  2:360724/139‑1 (MQ=255)
                                                                                                      ttttACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTggg                              >  1:175700/1‑138 (MQ=255)
                                                                                                         tACCATATTATCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCAt                           <  1:58285/138‑1 (MQ=255)
                                                                                                              tattatCTCGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCaa                      >  1:421151/1‑138 (MQ=255)
                                                                                                                      cGACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCa             >  1:457883/1‑139 (MQ=255)
                                                                                                                       gACTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCa             <  2:175700/138‑1 (MQ=255)
                                                                                                                        aCTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAat                                                                     <  2:294782/81‑1 (MQ=255)
                                                                                                                        aCTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAat                                                                     >  1:294782/1‑81 (MQ=255)
                                                                                                                        aCTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATa           <  1:314450/139‑1 (MQ=255)
                                                                                                                        aCTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATa           <  2:315047/139‑1 (MQ=255)
                                                                                                                        aCTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATa           <  1:547204/139‑1 (MQ=255)
                                                                                                                        aCTTCCGGTGGTAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATa           >  1:267671/1‑139 (MQ=255)
                                                                                                                                  gtAATACCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGt  <  2:421151/138‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CAGGCTTTAAAAAACTGGTTGAGTATCCGTACTTCGTACCCGCATGCTGAGAGCGAGTGGGTATTTTTATCACGTAAGGGGAATCCGCTTTCTCGGCAACAGTTTTACCATATTATCTCGACTTCCGGTGGTAATGCCGGGTTGTCACTGGAGATTCATCCGCACATGTTACGCCATTCGTGTGGTTTTGCTTTGGCGAATATGGGAATAGATACGCGACTTATCCAGGATTATCTTGGGCATCGCAATATTCGTCATACTGTCTGGT  >  NZ_CP009273/4531032‑4531299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑