Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,568,721	A→G	I145T (ATC→ACC)	mcrB ←	5‑methylcytosine‑specific restriction endonuclease subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,568,721	0	A	G	100.0%	139.4 / NA	37	I145T (ATC→ACC)	mcrB	5‑methylcytosine‑specific restriction endonuclease subunit McrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/18);  total (19/18)

CGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGTTT  >  NZ_CP009273/4568597‑4568856                                                                                                                             |                                                                                                                                        cGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                    >  2:405427/1‑130 (MQ=255) cGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTAtg                                                                                                                                    <  1:405427/130‑1 (MQ=255)              gTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATc                                                                                                              >  2:171348/1‑139 (MQ=255)                 tCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCggg                                                                                                           >  2:35316/1‑139 (MQ=255)                 tCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCggg                                                                                                           >  1:497304/1‑139 (MQ=255)                 tCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCgg                                                                                                            <  2:197740/138‑1 (MQ=255)                   aGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGgt                                                                                                          <  2:396537/138‑1 (MQ=255)                   aGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGgt                                                                                                          <  1:452550/138‑1 (MQ=255)                    gggATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGgtgt                                                                                                        <  2:562643/139‑1 (MQ=255)                    gggATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGgtgt                                                                                                        <  2:242637/139‑1 (MQ=255)                                                 cAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGaa                                                                           <  1:258371/139‑1 (MQ=255)                                                     cAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg                                                                       <  2:458148/139‑1 (MQ=255)                                                     cAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAgg                                                                       <  2:200378/139‑1 (MQ=255)                                                      aGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGAc                                                                                     >  1:228499/1‑124 (MQ=255)                                                      aGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGAc                                                                                     <  2:228499/124‑1 (MQ=255)                                                       gTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg                                                                     >  2:383452/1‑139 (MQ=255)                                                        tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATa                                                                                                <  1:277078/111‑1 (MQ=255)                                                        tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATa                                                                                                >  2:277078/1‑111 (MQ=255)                                                        tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg                                                                     >  1:248223/1‑138 (MQ=255)                                                        tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg                                                                     >  2:36882/1‑138 (MQ=255)                                                        tATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCg                                                                     >  2:438560/1‑138 (MQ=255)                                                             ttCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAg                                                               <  1:171348/139‑1 (MQ=255)                                                                aGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGAc                                                            >  2:393937/1‑139 (MQ=255)                                                                  tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAAt                                                                                                     >  1:456440/1‑96 (MQ=255)                                                                  tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAAt                                                                                                     <  2:456440/96‑1 (MQ=255)                                                                  tttttGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTg                                                          >  2:485401/1‑139 (MQ=255)                                                                            aGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAAt                                                                                                               >  2:158055/1‑76 (MQ=255)                                                                            aGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAAt                                                                                                               <  1:158055/76‑1 (MQ=255)                                                                                   aTAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGa                                          >  1:199925/1‑138 (MQ=255)                                                                                            ttGCCAGAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACAccc                                 >  2:197822/1‑138 (MQ=255)                                                                                                  gAATTAAATATTAATTTATAGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTg                          <  2:140134/139‑1 (MQ=255)                                                                                                                    taGTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGa        <  1:197822/139‑1 (MQ=255)                                                                                                                      gTCGTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGAt       <  2:199925/138‑1 (MQ=255)                                                                                                                         gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGAt       <  1:558442/135‑1 (MQ=255)                                                                                                                         gTTGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGAt       >  2:558442/1‑135 (MQ=255)                                                                                                                          ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGttt  >  1:340326/1‑139 (MQ=255)                                                                                                                          ttGGTTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGttt  >  2:500770/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                        CGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATTCAGTTTTTGACATAGGTGGAATAACACTCTTGCCAGAATTAAATATTAATTTATAGTCGTTGATTATGTTGTCCAGCATAGAGGCAAATCGGGTGTAATCAATACCCTGTGAGACTTTTTGGGAACAGGCGTAATAGGACTGTCCGTATTTTTTAGGATATACACCCGAAGTTGCCTGAAAATACTCTGCGATTGTTT  >  NZ_CP009273/4568597‑4568856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑