Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	487,218	A→C	K117N (AAA→AAC)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	487,218	0	A	C	100.0%	104.9 / NA	30	K117N (AAA→AAC)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/16);  total (14/16)

GCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTG  >  NZ_CP009273/487090‑487341                                                                                                                                 |                                                                                                                            gCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGAc                                                                                                                    <  1:550312/138‑1 (MQ=255) gCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACa                                                                                                                   <  2:575580/139‑1 (MQ=255)             ggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCtggtgg                                                                                                       >  2:562720/1‑139 (MQ=255)             ggCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCtgg                                                                                                          <  2:460039/136‑1 (MQ=255)                   ggCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGgac                                                                                                  >  2:159065/1‑138 (MQ=255)                              ggTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGc                                                                                       >  2:524080/1‑138 (MQ=255)                               gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGgacgac                                                                                               <  2:549433/129‑1 (MQ=255)                               gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGgacgac                                                                                               >  1:549433/1‑129 (MQ=255)                               gTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCaa                                                                                     >  2:158562/1‑139 (MQ=255)                                  cGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcg                                                                                  >  2:572131/1‑139 (MQ=255)                                   gTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcgg                                                                                 <  2:460785/139‑1 (MQ=255)                                    tAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcggc                                                                                <  1:538518/139‑1 (MQ=255)                                      aaCCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                              <  2:48674/139‑1 (MQ=255)                                        ccGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCa                                                                            <  1:159065/139‑1 (MQ=255)                                          gggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCAc                                                                           >  2:275341/1‑138 (MQ=255)                                          gggCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCAc                                                                           >  2:435739/1‑138 (MQ=255)                                                    ccGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                  <  2:469583/137‑1 (MQ=255)                                                    ccGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGc                                                                  <  1:524080/137‑1 (MQ=255)                                                      gcgTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                              <  2:264098/123‑1 (MQ=255)                                                      gcgTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACcggcgg                                                                              >  1:264098/1‑123 (MQ=255)                                                                 tCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACAGGCGGCACTATCGAAGCGACCGTTAAACTGAt                                                   >  2:112066/1‑139 (MQ=255)                                                                        aaCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCcgtcgt                                            <  2:334611/139‑1 (MQ=255)                                                                                      aaTACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGt                              <  2:149537/139‑1 (MQ=255)                                                                                           ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCt                          >  1:63554/1‑138 (MQ=255)                                                                                             cACCGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGAc                       >  1:41404/1‑139 (MQ=255)                                                                                                cGATCAGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCt                    <  1:158562/139‑1 (MQ=255)                                                                                                     aGCTGGAGATCCACGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGtt               <  2:41404/139‑1 (MQ=255)                                                                                                                  cGTTGATGCCATCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTg  >  1:266313/1‑139 (MQ=255)                                                                                                                             tCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGgtggtg                                  >  2:411169/1‑96 (MQ=255)                                                                                                                             tCAACCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGgtggtg                                  <  1:411169/96‑1 (MQ=255)                                                                                                                                 |                                                                                                                            GCTCTGGGTCTGGGCGTTGGCTTTGTACCGGTCCGTAAACCGGGCAAACTGCCGCGTGAAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTG  >  NZ_CP009273/487090‑487341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑