| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 490,029 | T→G | C57G (TGC→GGC) | recR → | recombination mediator RecR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 490,029 | 0 | T | G | 95.0% | 47.7 / ‑6.2 | 20 | C57G (TGC→GGC) | recR | recombination mediator RecR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (11/8); minor base C (1/0); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
CACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTT > NZ_CP009273/489898‑490164 | cACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCCGGCGCTCACCCGGGCGGTGTTGGGAATCGGCCACGGCGCCGa > 2:271276/1‑139 (MQ=255)cACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCCCACCCCGGCGATGTCGGGAATACGCCACCGCGcccc > 2:349852/1‑137 (MQ=255) cgcTGTCTGCCGGGCGTTGGCCCGAAGTTGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCCCCCGGGCGATGTCGGGAATCGGCCACGGCGCCGGGTGcc > 2:452148/1‑139 (MQ=255) cTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCCGGGGCCGGCGCTCACCCGGGCGGTGTCGGGAAACGGCCACGGCGCCGCTTGCCGCACtt > 2:145900/1‑139 (MQ=255) cgcAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGAGGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGGAATTCGCCACGGCGCCGGGTGCCGCACTTTCCACGCACAGGAGGTCTggtacaa > 2:528789/1‑133 (MQ=255) tCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCCGGCGCTCACCCCGGCGGTGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCcgcg > 1:356926/1‑139 (MQ=255) tCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCcccg > 1:508798/1‑139 (MQ=255) aGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGaaa < 2:508798/138‑1 (MQ=255) gTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAAt < 1:528789/139‑1 (MQ=255) gcAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCcggcg < 2:356926/139‑1 (MQ=255) aCCCGGGCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACg < 1:452148/139‑1 (MQ=255) gggCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCAt > 1:195593/1‑139 (MQ=255) ggCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGt < 2:330485/97‑1 (MQ=255) ggCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGt > 1:330485/1‑97 (MQ=255) ggCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCt > 1:350964/1‑104 (MQ=255) ggCGATGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCt < 2:350964/104‑1 (MQ=255) tGTCGGAAATCGGCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAg > 2:185043/1‑137 (MQ=255) ggCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGa > 1:572810/1‑130 (MQ=255) ggCCACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGa < 2:572810/130‑1 (MQ=255) cACGGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGtttt < 1:349852/139‑1 (MQ=255) | CACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAATCGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAACGGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTT > NZ_CP009273/489898‑490164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |