Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	506,540	T→C	D99G (GAC→GGC)	copA ←	copper‑exporting P‑type ATPase CopA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	506,540	0	T	C	100.0%	130.7 / NA	38	D99G (GAC→GGC)	copA	copper‑exporting P‑type ATPase CopA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

GCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGATGCGTCATAACCCGCTTGTTTGATGGTT  >  NZ_CP009273/506418‑506674                                                                                                                           |                                                                                                                                       gcgcAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGt                                                                                                                        >  2:55864/1‑139 (MQ=255)      gTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTg                                                                                                                   >  1:109552/1‑139 (MQ=255)      gTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTg                                                                                                                   >  1:258453/1‑139 (MQ=255)            tCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCgg                                                                                                               >  1:446516/1‑137 (MQ=255)            tCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCCGCCgg                                                                                                               >  2:230057/1‑137 (MQ=255)             ccGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAg                                                                                                            >  1:88718/1‑139 (MQ=255)              cGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAgc                                                                                                           <  1:383470/139‑1 (MQ=255)               gCCAGGTTTACCCGTACCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCCGCCGGGAgcg                                                                                                          >  2:198900/1‑139 (MQ=255)                        aCCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGaa                                                                                                  >  2:327078/1‑138 (MQ=255)                                           ccGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGa                                                                              <  1:305915/139‑1 (MQ=255)                                           ccGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGATGGCGCAGCTCATGCCGCTGAGCAGCAACTGCTGGGTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGa                                                                              <  1:351141/139‑1 (MQ=255)                                                    tttGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCCGAAACCGCCGGCAGTGCTTCCGACGGGGTtgg                                                                     >  2:240304/1‑138 (MQ=255)                                                     ttGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCCCCCGGAAACCCCTCACAAACCCCTGTCAGTTCTTCCCCCGGGACtggt                                                                    >  1:316246/1‑137 (MQ=255)                                                      tGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATtgat                                                                    <  1:327078/138‑1 (MQ=255)                                                           cgcATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAcc                                                                                               <  2:51484/106‑1 (MQ=255)                                                           cgcATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAcc                                                                                               >  1:51484/1‑106 (MQ=255)                                                           cgcATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAcc                                                                                               <  2:176930/106‑1 (MQ=255)                                                           cgcATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAAcc                                                                                               >  1:176930/1‑106 (MQ=255)                                                             cATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGa                                                                              >  1:507767/1‑121 (MQ=255)                                                             cATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGa                                                                              <  2:507767/121‑1 (MQ=255)                                                               ttttGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCa                                                          <  2:258453/139‑1 (MQ=255)                                                                          ggTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGCAGCGCCTCCGAAACCGCCGTCAGTGCTTCCGGCGGGGTTGATGACTCCGCCAGCGGCTTAGcc                                               >  2:131115/1‑139 (MQ=255)                                                                            tGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTgcca                                                                                                                                      <  1:170426/50‑1 (MQ=255)                                                                            tGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTgcca                                                                                                                                      >  2:170426/1‑50 (MQ=255)                                                                            tGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCtt                                             >  1:527563/1‑139 (MQ=255)                                                                            tGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGGCGCTGCCGGAAGGGCCCCAGGAACCGCTGTCAGTGGTTTCGACCGGGGTGTTGGCTCCGCCAGCGGGTTTGGCtt                                             >  2:499688/1‑139 (MQ=255)                                                                                acaGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg                                         <  2:109552/139‑1 (MQ=255)                                                                                acaGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg                                         <  1:240304/139‑1 (MQ=255)                                                                                acaGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTggg                                         <  2:24788/139‑1 (MQ=255)                                                                                   gCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGGAGCGCCTCCGAAACCGCTGTCAGTGCTTCCGGCGGGGTTGGTGACTCCGCCAGCGGTTTTGCCTTTgggggg                                      >  2:84391/1‑139 (MQ=255)                                                                                     tGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCCGAAACGCCTCAGAAACCGCTGTCAGCGCTTTCGGCGGGGTTGTTGACTCCGCCAGCGGCTTAGCCTTTGGGGGGCt                                    >  2:534453/1‑139 (MQ=255)                                                                                            gCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGAt                             <  1:84391/139‑1 (MQ=255)                                                                                            gCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGAt                             <  1:397769/139‑1 (MQ=255)                                                                                            gCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGAt                             <  2:88718/139‑1 (MQ=255)                                                                                            gCTCATGCCGCTCAGCAGCAACTGCTGGCTGCCATCGTCATCGGCGGTCCCTGCCGGCAGGGCCTCCGAAACCCCCGTCAGTGCTTCCGGCGGGGTTGCTGGATCCGCCAGCGGGTTTGCCTTTTGGGGGCTTACAg                               >  2:397769/1‑137 (MQ=255)                                                                                                           cagcaACTGCTGGCTGCCATCGTCATCGGCGGTCGCTGCCGGCAGCGCCTCCGACACCGCTGTCCCTGCTTCCGTCCGGCTTGCTGACTCCCCCCGCGGTTTTGCCTTTGGGTGGCTTTCAGATGCCTCATAACCCCCt              >  2:74559/1‑139 (MQ=255)                                                                                                                aCTGCTGGCTGCCATCGTCATCGGCGGTCGCCGCCGGGAGAGCCTCAGGAACCGCTGTCAGTGCTTCCGCCGGGATTGGTGAAGCCGCCCGCGGATTTGGCTTTTGGGGGGTTTCAGATGATGTACAACCCGCCTGttt         >  2:535922/1‑139 (MQ=255)                                                                                                                   gctggctgCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCCCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGATGCGTCATAAACCGCTTGTTTGAt      >  2:402188/1‑139 (MQ=255)                                                                                                                    ctggctgCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGATGCGTCATAACCCGCTTGTTTGa       <  2:316246/137‑1 (MQ=255)                                                                                                                       gctgCCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGATGCGTCATAACCCGCTTGTTTGATGGtt  <  1:55864/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                       GCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCTCAGAAACCGCTGTCAGTGCTTCCGACGGGATTGATGACTCCGCCAGCGGTTTAGCCTTTGGGTGGCTTACAGATGCGTCATAACCCGCTTGTTTGATGGTT  >  NZ_CP009273/506418‑506674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑