Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	527,133	A→G	V182A (GTT→GCT)	allS ←	HTH‑type transcriptional activator AllS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	527,133	0	A	G	100.0%	130.4 / NA	36	V182A (GTT→GCT)	allS	HTH‑type transcriptional activator AllS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

TCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAA  >  NZ_CP009273/527007‑527263                                                                                                                               |                                                                                                                                   tCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGc                                                                                                                         <  1:370164/138‑1 (MQ=255)                   ttttGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGa                                                                                                      >  2:200385/1‑139 (MQ=255)                      tGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACa                                                                                                    <  1:101304/138‑1 (MQ=255)                        cccTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAg                                                                                                 <  1:355823/139‑1 (MQ=255)                            ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcgc                                                                                              >  2:339006/1‑138 (MQ=255)                            ggCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAgcgc                                                                                              >  1:560154/1‑138 (MQ=255)                              cAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCAt                                                                                           <  2:235525/139‑1 (MQ=255)                                   gCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAc                                                                                      <  2:78462/139‑1 (MQ=255)                                   gCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAc                                                                                      <  1:339006/139‑1 (MQ=255)                                    ccAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc                                                                                     <  1:147460/139‑1 (MQ=255)                                    ccAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCAcc                                                                                     <  2:560154/139‑1 (MQ=255)                                       ggCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGAt                                                                                  <  2:260978/139‑1 (MQ=255)                                       ggCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGAt                                                                                  >  1:505249/1‑139 (MQ=255)                                             gcgTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAg                                                                            >  1:284350/1‑139 (MQ=255)                                                             cGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAgg                                                            >  1:144775/1‑139 (MQ=255)                                                              gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTaa                                                               >  1:401761/1‑135 (MQ=255)                                                              gggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTaa                                                               <  2:401761/135‑1 (MQ=255)                                                               ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCa                                                                                            <  1:483135/105‑1 (MQ=255)                                                               ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCa                                                                                            >  2:483135/1‑105 (MQ=255)                                                               ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa                                                                             >  2:296125/1‑120 (MQ=255)                                                               ggCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTaa                                                                             <  1:296125/120‑1 (MQ=255)                                                                   cTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTg                                                      >  2:157688/1‑139 (MQ=255)                                                                          cAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAg                                                  <  2:447075/136‑1 (MQ=255)                                                                           aaTATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               <  1:421790/138‑1 (MQ=255)                                                                           aaTATTGACCGCCGGAAAGCGCCACAACTGCGCTTCTGTTAGCGGCTTTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAACGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               <  2:392345/138‑1 (MQ=255)                                                                                tGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACa                                                                                                    >  1:289822/1‑80 (MQ=255)                                                                                tGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACa                                                                                                    <  2:289822/80‑1 (MQ=255)                                                                                     gccgGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCg                                    >  1:195037/1‑139 (MQ=255)                                                                                                                 ttAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCt          <  1:157688/137‑1 (MQ=255)                                                                                                                 ttAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCt          <  2:424796/137‑1 (MQ=255)                                                                                                                 ttAGCGGCTCTTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTc        >  1:424796/1‑139 (MQ=255)                                                                                                                        ctctTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               <  2:274708/93‑1 (MQ=255)                                                                                                                        ctctTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAgcgc                                               >  1:274708/1‑93 (MQ=255)                                                                                                                         tctTCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACaa  >  2:6868/1‑137 (MQ=255)                                                                                                                           ttCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAg                    >  2:219531/1‑117 (MQ=255)                                                                                                                           ttCAGCGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAg                    <  1:219531/117‑1 (MQ=255)                                                                                                                               |                                                                                                                                   TCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTCAATATTGACCGCCGGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGCCGCCATGACAAAGCGCCATTGCACCGATCCTAAGGGATCAAGACTAAAGGTATTTGCCAGCGCCTCAGTTCCCGTGACGCCGATAGCCAGCGAAAAACCTTCGTACAA  >  NZ_CP009273/527007‑527263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑