Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	552,665	T→C	E178G (GAA→GGA)	folD ←	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	552,665	0	T	C	100.0%	102.8 / NA	30	E178G (GAA→GGA)	folD	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/18);  total (12/18)

CTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGA  >  NZ_CP009273/552531‑552787                                                                                                                                       |                                                                                                                           cttTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCa                                                                                                                         <  1:92726/138‑1 (MQ=255)          aGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCgg                                                                                                               >  2:156316/1‑139 (MQ=255)            tCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGggc                                                                                                             >  1:139503/1‑139 (MQ=255)                 gggAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCa                                                                                                        <  1:51209/139‑1 (MQ=255)                                          gCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATcacca                                                                               >  2:119388/1‑139 (MQ=255)                                            aaCGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACg                                                                             <  2:351415/139‑1 (MQ=255)                                             aCGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACgg                                                                            >  1:467357/1‑139 (MQ=255)                                                 tCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGtt                                                                        <  2:139503/139‑1 (MQ=255)                                                 acAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGtt                                                                        <  1:196571/138‑1 (MQ=255)                                                         tCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGa                                                                <  2:467357/139‑1 (MQ=255)                                                          cGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCggg                                                                                                              <  1:311174/92‑1 (MQ=255)                                                          cGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCggg                                                                                                              >  2:311174/1‑92 (MQ=255)                                                          cGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCg                                                                 >  1:30390/1‑137 (MQ=255)                                                           ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGaa                                                               <  1:156316/138‑1 (MQ=255)                                                           ggCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAg                                                              >  2:430691/1‑139 (MQ=255)                                                                                   aTTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAagc                                      <  2:227619/139‑1 (MQ=255)                                                                                        tAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCg                                  <  1:78634/138‑1 (MQ=255)                                                                                             aaGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTAt                                                          <  2:195524/109‑1 (MQ=255)                                                                                             aaGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTAt                                                          >  1:195524/1‑109 (MQ=255)                                                                                                cGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATAc                         >  2:95256/1‑139 (MQ=255)                                                                                                      gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                   <  1:551594/139‑1 (MQ=255)                                                                                                      gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                   <  2:30390/139‑1 (MQ=255)                                                                                                      gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                   <  1:291759/139‑1 (MQ=255)                                                                                                      gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                   <  1:257303/139‑1 (MQ=255)                                                                                                      gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                   <  1:188626/139‑1 (MQ=255)                                                                                                      gTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCAAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACcgcgcg                   <  1:388995/139‑1 (MQ=255)                                                                                                                       aCCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACg                                             <  1:472326/96‑1 (MQ=255)                                                                                                                       aCCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACg                                             >  2:472326/1‑96 (MQ=255)                                                                                                                       aCCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGa  >  1:516570/1‑139 (MQ=255)                                                                                                                       aCCTGCCAGCAGCAGTCCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGa  >  1:130641/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                           CTTTGATCCAGTCACCGGGAATAAAGCCTGGCTTGCCAACGGCAACGATCAATAGATCGGCATTTTCTACGTGATGACGCAGATTTTTAGTGAAGCGGTGAGTCACTGTAGTGGTGCAACCTGCCAGCAGCAGTTCCATGCTCATCGGGCGGCCAACGATATTCGATGCGCCAATCACCACGGCGTTGAGGCCGAAGGTATCAATGTTGTAACGCTCAAGCAGCGTGACGATACCGCGCGGGGTGCAGGGACGCAGA  >  NZ_CP009273/552531‑552787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑