Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	587,628	A→G	F336S (TTC→TCC)	nrfB ←	cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	587,628	0	A	G	100.0%	120.9 / NA	32	F336S (TTC→TCC)	nrfB	cyclic di‑3',5'‑guanylate‑activated glycosyltransferase NrfB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

GGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGAAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTAAAAATTTACGCTGCTCGCG  >  NZ_CP009273/587509‑587752                                                                                                                        |                                                                                                                             ggATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATc                                                                                                             >  1:20989/1‑137 (MQ=255)    tcatcaCCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCg                                                                                                        <  2:20989/139‑1 (MQ=255)             gCATCGCGAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTg                                                                                               >  2:153267/1‑139 (MQ=255)             gCATCGCGAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTg                                                                                               >  2:127106/1‑139 (MQ=255)                 cgcgAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCg                                                                                            >  2:370014/1‑138 (MQ=255)                  gcgAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGaa                                                                                          >  2:239455/1‑139 (MQ=255)                   cgAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAAc                                                                                         >  1:137321/1‑139 (MQ=255)                   cgAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAAc                                                                                         >  2:474099/1‑139 (MQ=255)                     aGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCg                                                                                       >  1:551406/1‑139 (MQ=255)                      ggAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGc                                                                                      <  1:474099/139‑1 (MQ=255)                      ggAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGc                                                                                      <  1:126096/139‑1 (MQ=255)                              aCAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGaaa                                                                              <  1:260467/139‑1 (MQ=255)                              aCAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGaaa                                                                              <  1:370014/139‑1 (MQ=255)                              aCAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGaaa                                                                              <  2:567635/139‑1 (MQ=255)                                                  tttGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTcg                                                          >  1:331030/1‑139 (MQ=255)                                                        gTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGcgcacg                                                    <  2:109331/139‑1 (MQ=255)                                                        gTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGcgcacg                                                    <  1:427454/139‑1 (MQ=255)                                                          cgcgCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGcgcacg                                                    >  1:433224/1‑137 (MQ=255)                                                             gcCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGAt                                               <  1:196195/139‑1 (MQ=255)                                                             gcCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGAt                                               <  2:458503/139‑1 (MQ=255)                                                                       gtagtTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGcgcacg                                                    >  1:529404/1‑124 (MQ=255)                                                                       gtagtTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGcgcacg                                                    <  2:529404/124‑1 (MQ=255)                                                                         agtTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGt                                   >  1:474502/1‑139 (MQ=255)                                                                           ttCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTcc                                 >  1:496501/1‑139 (MQ=255)                                                                           ttCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTcc                                 >  2:286889/1‑139 (MQ=255)                                                                                  tCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTg                          >  1:436717/1‑139 (MQ=255)                                                                                  tCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTg                          >  2:363571/1‑139 (MQ=255)                                                                                       cTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTa                     <  2:433224/139‑1 (MQ=255)                                                                                               ccATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTAAAAATTTa             >  1:263371/1‑139 (MQ=255)                                                                                                cATTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTAAAAATTTAc            >  1:538554/1‑139 (MQ=255)                                                                                                 aTTTATGGGTTTTAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTAAAAATTTACg           <  2:474502/139‑1 (MQ=255)                                                                                                          ttttAAAGCCTTGGGAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTAAAAATTTACGCTGCTcgcg  <  2:178396/139‑1 (MQ=255)                                                                                                                        |                                                                                                                             GGATCATCACCAGCATCGCGAGGAAGCTGACAAAGTTACTGATTGCCCCTTTGCGGTCGCGCCAGAGAAAGTAGTTCAGCGTCAGGCTGGAGGTCCATTTATGGGTTTTAAAGCCTTGGAAAACAATGCCGATGATCCAGCGGGATTTTTGTCGAACCGCAGTCGAAAAGGTATCGGGGAAATATTCGCGCACGCAGATCATGTTTGATGTCCGCGCGTGCTGTAAAAATTTACGCTGCTCGCG  >  NZ_CP009273/587509‑587752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑