Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	592,602	C→T	Q6* (CAA→TAA)	cusF →	Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	592,602	0	C	T	100.0%	128.5 / NA	32	Q6* (CAA→TAA)	cusF	Cu(+)/Ag(+) efflux RND transporter periplasmic metallochaperone CusF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

CGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAAAGGGTA  >  NZ_CP009273/592468‑592725                                                                                                                                       |                                                                                                                            cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAg                                                                                                                          <  2:15452/138‑1 (MQ=255)  gtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTc                                                                                                                        <  1:566454/139‑1 (MQ=255)            gCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTc                                                                                                               <  2:343352/138‑1 (MQ=255)             cTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAg                                                                                                             <  1:541258/139‑1 (MQ=255)                aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGt                                                                                                            <  2:56110/137‑1 (MQ=255)                aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGt                                                                                                            <  1:381508/137‑1 (MQ=255)                     aGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTa                                                                                                     <  2:364579/139‑1 (MQ=255)                      gTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTAc                                                                                                    >  1:448039/1‑139 (MQ=255)                            tacttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTAt                                                                                              >  1:133047/1‑139 (MQ=255)                             acttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTa                                                                                               <  2:545320/137‑1 (MQ=255)                                 aTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCt                                                                                         >  2:240963/1‑139 (MQ=255)                                 aTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCt                                                                                         >  1:576390/1‑139 (MQ=255)                                   gTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCtt                                                                                        >  1:156327/1‑138 (MQ=255)                                        cTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGc                                                                                  >  1:260922/1‑139 (MQ=255)                                         tCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGcc                                                                                 >  1:256060/1‑139 (MQ=255)                                              gggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAgg                                                                             <  2:61296/138‑1 (MQ=255)                                                    cACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACg                                                                       <  2:133047/138‑1 (MQ=255)                                                     aCTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGc                                                                                                                      <  2:334349/90‑1 (MQ=255)                                                     aCTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGc                                                                                                                      >  1:334349/1‑90 (MQ=255)                                                     aCTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGaa                                                                     >  2:129769/1‑139 (MQ=255)                                                             gTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcatcatca                                                             >  1:444567/1‑139 (MQ=255)                                                                     ccTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAAcc                                                      <  2:156327/138‑1 (MQ=255)                                                                         cAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAg                                                 <  2:444567/139‑1 (MQ=255)                                                                            cTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGa                                              <  2:260922/139‑1 (MQ=255)                                                                                aaTTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGca                                          <  1:192230/139‑1 (MQ=255)                                                                                       tAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACca                                    >  2:518273/1‑138 (MQ=255)                                                                                                    ttAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcat                                                                  >  1:575046/1‑95 (MQ=255)                                                                                                    ttAACTCCAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAAcat                                                                  <  2:575046/95‑1 (MQ=255)                                                                                                           cAGGAGAGAATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGc               <  1:518273/139‑1 (MQ=255)                                                                                                                 agaATAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTa         <  1:520119/139‑1 (MQ=255)                                                                                                                   aaTAAATGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTa         >  1:131385/1‑137 (MQ=255)                                                                                                                        aTGAAAAAAGCACTGTAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAAAGGGTa  <  2:131385/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                            CGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGACTTTTAACTCCAGGAGAGAATAAATGAAAAAAGCACTGCAAGTCGCAATGTTCAGTCTGTTTACCGTTATTGGCTTTAATGCCCAGGCTAACGAACATCATCATGAAACCATGAGCGAAGCACAACCACAGGTTATTAGCGCCACTGGCGTGGTAAAGGGTA  >  NZ_CP009273/592468‑592725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑