Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	736,034	A→T	I358F (ATC→TTC)	phrB →	deoxyribodipyrimidine photo‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	736,034	0	A	T	100.0%	136.3 / NA	34	I358F (ATC→TTC)	phrB	deoxyribodipyrimidine photo‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/15);  total (19/15)

ACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGATCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGA  >  NZ_CP009273/735904‑736169                                                                                                                                   |                                                                                                                                        aCAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGACAGATTTATTGTTCGAc                                                                                                                                    >  1:521359/1‑136 (MQ=255) aCAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGAc                                                                                                                                    <  2:521359/136‑1 (MQ=255)             ggAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGgcgag                                                                                                                     <  2:222733/139‑1 (MQ=255)                       aCGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCa                                                                                                           <  1:258035/139‑1 (MQ=255)                            aTACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCGTGTCg                                                                                                      >  1:315086/1‑139 (MQ=255)                               cccGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAg                                                                                                   <  2:557882/139‑1 (MQ=255)                                         gATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCtgattgat                                                                                          <  1:148251/138‑1 (MQ=255)                                                               aCAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACg                                                                    <  2:905/138‑1 (MQ=255)                                                                     ctggctggATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATAGTGATTTGGCAGCCAATAACGGtggctg                                                             >  1:560101/1‑139 (MQ=255)                                                                        ggtggATGCATAACAGGCGACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGCGATTTGGCAGCCAATAACGGtggctgg                                                            >  2:158394/3‑137 (MQ=255)                                                                        gctggATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTgg                                                                                >  2:98080/1‑117 (MQ=255)                                                                        gctggATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTgg                                                                                <  1:98080/117‑1 (MQ=255)                                                                             aTGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCa                                                                              <  1:126785/114‑1 (MQ=255)                                                                             aTGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCa                                                                              >  2:126785/1‑114 (MQ=255)                                                                                  tAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCt                                                >  2:77705/1‑139 (MQ=255)                                                                                       ggCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTgg                                                                                >  2:305634/1‑102 (MQ=255)                                                                                       ggCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTgg                                                                                <  1:305634/102‑1 (MQ=255)                                                                                        gCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGtggc                                                               >  2:284399/1‑118 (MQ=255)                                                                                        gCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGtggc                                                               <  1:284399/118‑1 (MQ=255)                                                                                        gCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAAcc                                          >  1:236803/1‑139 (MQ=255)                                                                                          tACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCg                                                                                                      >  1:439108/1‑77 (MQ=255)                                                                                          tACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCg                                                                                                      <  2:439108/77‑1 (MQ=255)                                                                                          tACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAAcc                                          >  2:484415/1‑137 (MQ=255)                                                                                          tACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAAcc                                          <  1:484415/137‑1 (MQ=255)                                                                                            cGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGaa                                      >  2:501517/1‑139 (MQ=255)                                                                                             ggATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCGTGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAAc                                     <  2:315086/139‑1 (MQ=255)                                                                                                  atTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATg                                >  1:537653/1‑139 (MQ=255)                                                                                                  atTACAGCCAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATg                                >  1:97124/1‑139 (MQ=255)                                                                                                         ccAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGcc                         >  2:146449/1‑139 (MQ=255)                                                                                                         ccAGTTTTCTGGTGAAAGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGcc                         >  1:19833/1‑139 (MQ=255)                                                                                                                       aaaGATTTATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCcgtattttcgtattt           >  1:394330/1‑139 (MQ=255)                                                                                                                              tATTGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAAccc    >  1:572929/1‑139 (MQ=255)                                                                                                                                ttGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGa  <  2:236695/139‑1 (MQ=255)                                                                                                                                ttGTTCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGa  <  2:236803/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        ACAGGCCTGGCAGGAAGGCAAAACGGGATACCCGATTGTTGATGCCGCTATGCGTCAGCTTAACAGCACTGGCTGGATGCATAACAGGCTACGGATGATTACAGCCAGTTTTCTGGTGAAAGATTTATTGATCGACTGGCGCGAAGGCGAGCGATATTTCATGTCGCAGCTGATTGATGGTGATTTGGCAGCCAATAACGGTGGCTGGCAGTGGGCCGCTTCAACCGGAACCGATGCAGCGCCGTATTTTCGTATTTTCAACCCGA  >  NZ_CP009273/735904‑736169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑