Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	737,899	A→G	N38N (AAT→AAC)	dtpD ←	dipeptide permease DtpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	737,899	0	A	G	100.0%	134.5 / NA	37	N38N (AAT→AAC)	dtpD	dipeptide permease DtpD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

CCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAAT  >  NZ_CP009273/737766‑738015                                                                                                                                      |                                                                                                                     cccccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTtggttggt                                                                                                                 <  2:172621/139‑1 (MQ=255) cccccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTtggttggt                                                                                                                 <  1:572765/139‑1 (MQ=255)    ccAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTtggttggt                                                                                                                 >  1:336104/1‑136 (MQ=255)             ccGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAAt                                                                                                     <  2:379421/139‑1 (MQ=255)                ccATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATcag                                                                                                  <  1:156955/139‑1 (MQ=255)                ccATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATcag                                                                                                  >  1:507650/1‑139 (MQ=255)                  aTGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAACTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATcag                                                                                                  >  2:371717/1‑137 (MQ=255)                           ccGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCa                                                                                       <  2:387704/139‑1 (MQ=255)                            cGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCAt                                                                                      <  2:580127/139‑1 (MQ=255)                             gagaACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATg                                                                                     >  2:448866/1‑139 (MQ=255)                                     tATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTaa                                                                               >  2:562740/1‑137 (MQ=255)                                                           gATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGc                                                       <  1:178105/139‑1 (MQ=255)                                                                  gTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCaa                                                <  2:50842/139‑1 (MQ=255)                                                                     aCATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                              <  2:507650/138‑1 (MQ=255)                                                                      cATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGAc                                            <  2:336104/139‑1 (MQ=255)                                                                               gCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                    >  2:184475/1‑138 (MQ=255)                                                                               gCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                    >  2:469007/1‑138 (MQ=255)                                                                                cGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                    >  2:377394/1‑137 (MQ=255)                                                                                 gAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAg                                 >  2:15163/1‑139 (MQ=255)                                                                                  aaCAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcg                                                   >  1:433924/1‑120 (MQ=255)                                                                                  aaCAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAgcg                                                   <  2:433924/120‑1 (MQ=255)                                                                                  aaCAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGAc                                            <  1:359302/127‑1 (MQ=255)                                                                                  aaCAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGAc                                            >  2:359302/1‑127 (MQ=255)                                                                                     aGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg                             <  1:184475/139‑1 (MQ=255)                                                                                     aGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg                             <  1:562740/139‑1 (MQ=255)                                                                                       tAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGcgcgcg                           >  1:146346/1‑139 (MQ=255)                                                                                                aaTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATg                  <  1:552895/139‑1 (MQ=255)                                                                                                aaTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATg                  <  1:273288/139‑1 (MQ=255)                                                                                                 aTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAg                                                     >  1:290059/1‑103 (MQ=255)                                                                                                 aTAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAg                                                     <  2:290059/103‑1 (MQ=255)                                                                                                     cTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgtgt             >  1:75401/1‑139 (MQ=255)                                                                                                     cTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgtgt             <  2:146346/139‑1 (MQ=255)                                                                                                     cTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCAGCTGTGATGCgtgt             <  2:276524/139‑1 (MQ=255)                                                                                                          aGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                              <  2:309851/101‑1 (MQ=255)                                                                                                          aGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                              >  1:309851/1‑101 (MQ=255)                                                                                                          aGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTAtt        >  2:355026/1‑139 (MQ=255)                                                                                                          aGGCGTGAGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTAtt        >  1:507800/1‑139 (MQ=255)                                                                                                                 aGTATCGTTGTATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCataat  <  1:377394/138‑1 (MQ=255)                                                                                                                           tATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                              >  2:198064/1‑84 (MQ=255)                                                                                                                           tATTTTAGTTGGTTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACg                                              <  1:198064/84‑1 (MQ=255)                                                                                                                                      |                                                                                                                     CCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAGATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAAT  >  NZ_CP009273/737766‑738015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑