Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	771,555	A→G	E84G (GAG→GGG)	tolR →	colicin uptake protein TolR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	771,555	0	A	G	100.0%	96.1 / NA	29	E84G (GAG→GGG)	tolR	colicin uptake protein TolR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/11);  total (18/11)

CCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA  >  NZ_CP009273/771423‑771687                                                                                                                                     |                                                                                                                                     cccAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGttt                                                                                                                                <  2:485633/139‑1 (MQ=255)   cAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTac                                                                                                                              <  2:150624/139‑1 (MQ=255)      agCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTaccac                                                                                                                           >  1:263346/1‑139 (MQ=255)        cGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTaccac                                                                                                                           >  1:573467/1‑137 (MQ=255)                                     aaTCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGc                                                                                              <  2:429273/137‑1 (MQ=255)                                          caGGCGGTGCGCAGTAACGATAATCCGCCCGCGATTGTTGAAGTGTCTGGTATTGGTCAGGACACCGTGGTGGTTGAGCAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTc                                                                                        >  1:186519/1‑138 (MQ=255)                                          caGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTc                                                                                        >  1:50069/1‑138 (MQ=255)                                            ggCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAg                                                                                     >  1:319415/1‑139 (MQ=255)                                                             aTAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGt                                                                    >  1:159629/1‑139 (MQ=255)                                                              tAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTc                                                                   <  2:63960/139‑1 (MQ=255)                                                                            ttgttgAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTgg                                                     <  2:502535/139‑1 (MQ=255)                                                                               ttgAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGgcgc                                                  <  1:224706/139‑1 (MQ=255)                                                                                 gaggtgtCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGAGCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCa                                                 <  2:186519/135‑1 (MQ=255)                                                                                       tCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                           <  2:438070/138‑1 (MQ=255)                                                                                       tCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGAt                                           <  2:249050/138‑1 (MQ=255)                                                                                                 gTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGa                                >  1:47554/1‑139 (MQ=255)                                                                                                      tACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                           >  1:10727/1‑139 (MQ=255)                                                                                                      tACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                           >  2:240079/1‑139 (MQ=255)                                                                                                      tACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                           >  1:556735/1‑139 (MQ=255)                                                                                                      tACACCGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGaaa                           >  1:492285/1‑139 (MQ=255)                                                                                                           cGTGGTGGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAaataat                       >  1:553637/1‑138 (MQ=255)                                                                                                            gtggtgGTTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAAtt                      >  2:468424/1‑138 (MQ=255)                                                                                                                tggtTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAg                  >  2:204068/1‑138 (MQ=255)                                                                                                                tggtTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAg                  >  2:149410/1‑138 (MQ=255)                                                                                                                tggtTGAGAAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAg                  >  1:419542/1‑138 (MQ=255)                                                                                                                     gagaAAGATCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTg             >  1:195813/1‑138 (MQ=255)                                                                                                                            aTCGCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGt      >  1:286325/1‑138 (MQ=255)                                                                                                                               gCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa  <  2:159629/139‑1 (MQ=255)                                                                                                                               gCCTGGGGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACa  <  1:468424/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     CCCAGAGCGTGGAGGTCGATCTGCCAGACGCTACTGAATCACAGGCGGTGAGCAGTAACGATAATCCGCCAGTGATTGTTGAAGTGTCTGGTATTGGTCAGTACACCGTGGTGGTTGAGAAAGATCGCCTGGAGCGTTTACCACCAGAGCAGGTGGTGGCGGAAGTGTCCAGCCGTTTCAAGGCCAACCCGAAAACGGTCTTTCTGATCGGTGGCGCAAAAGATGTGCCTTACGATGAAATAATTAAAGCACTGAACTTGTTACA  >  NZ_CP009273/771423‑771687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑