Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	771,797	A→G	intergenic (+64/‑1)	tolR → / → tolA	colicin uptake protein TolR/cell envelope integrity protein TolA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	771,797	0	A	G	100.0%	122.9 / NA	35	intergenic (+64/‑1)	tolR/tolA	colicin uptake protein TolR/cell envelope integrity protein TolA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/19);  total (16/19)

AATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGA  >  NZ_CP009273/771661‑771926                                                                                                                                         |                                                                                                                                  aataatTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGgt                                                                                                                                 >  1:55531/1‑139 (MQ=255)        aaaGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCaaa                                                                                                                           <  1:224730/138‑1 (MQ=255)            cACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCaa                                                                                                                      <  2:404931/139‑1 (MQ=255)                 aaCTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGaa                                                                                                                 >  1:400784/1‑139 (MQ=255)                      gTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACaa                                                                                                              <  2:400784/137‑1 (MQ=255)                         aCATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACg                                                                                                          <  2:419542/138‑1 (MQ=255)                          cATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGAc                                                                                                        <  2:532429/139‑1 (MQ=255)                               tGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCt                                                                                                   >  2:361157/1‑139 (MQ=255)                                         aaaTCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCg                                                                                         >  2:181815/1‑139 (MQ=255)                                              ggttggttTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAat                                                                                    >  2:186192/1‑139 (MQ=255)                                                       aaTGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTcag                                                                            <  2:573467/138‑1 (MQ=255)                                                       aaTGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTcag                                                                            <  2:286325/138‑1 (MQ=255)                                                              cAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCaa                                                                                                                      <  1:393666/89‑1 (MQ=255)                                                              cAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCaa                                                                                                                      >  2:393666/1‑89 (MQ=255)                                                              cAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATa                                                                                      >  1:202190/1‑121 (MQ=255)                                                              cAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATa                                                                                      <  2:202190/121‑1 (MQ=255)                                                              cAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGtgctg                                                                    >  2:151066/1‑139 (MQ=255)                                                              cAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGtgctg                                                                    >  2:180469/1‑139 (MQ=255)                                                               aGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGtgctgc                                                                   <  2:99347/139‑1 (MQ=255)                                                                     tCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGt                                                               <  2:319415/137‑1 (MQ=255)                                                                              cTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTg                                                    >  1:365884/1‑139 (MQ=255)                                                                               tGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTgc                                                   >  2:429716/1‑139 (MQ=255)                                                                                                   gagagCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgt                               <  2:50069/139‑1 (MQ=255)                                                                                                      agCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAgttcgttc                             >  1:537588/1‑138 (MQ=255)                                                                                                        cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATa                                                                                      >  1:79977/1‑79 (MQ=255)                                                                                                        cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATa                                                                                      <  2:79977/79‑1 (MQ=255)                                                                                                        cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                          <  1:361157/139‑1 (MQ=255)                                                                                                        cGGGTAACAGGCGAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGAt                          <  2:12840/139‑1 (MQ=255)                                                                                                                    gAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTcag                                                                            >  1:66739/1‑77 (MQ=255)                                                                                                                    gAACAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTcag                                                                            <  2:66739/77‑1 (MQ=255)                                                                                                                     aaCAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTgcggcg                                               >  2:253795/1‑105 (MQ=255)                                                                                                                     aaCAGTTTTTGGAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTgcggcg                                               <  1:253795/105‑1 (MQ=255)                                                                                                                                gAAACCGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGa  <  1:180469/139‑1 (MQ=255)                                                                                                                                    ccGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTgcgg                                                 <  1:472364/88‑1 (MQ=255)                                                                                                                                    ccGAGGGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTgcgg                                                 >  2:472364/1‑88 (MQ=255)                                                                                                                                         |                                                                                                                                  AATAATTAAAGCACTGAACTTGTTACATAGTGCGGGTGTGAAATCGGTTGGTTTAATGACGCAGCCTATCTAAACATCTGCGTTTCCCTTGCTTGAAAGAGAGCGGGTAACAGGCGAACAGTTTTTGGAAACCGAGAGTGTCAAAGGCAACCGAACAAAACGACAAGCTCAAGCGGGCGATAATTATTTCAGCAGTGCTGCATGTCATCTTATTTGCGGCGCTGATCTGGAGTTCGTTCGATGAGAATATAGAAGCTTCAGCCGGA  >  NZ_CP009273/771661‑771926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑