Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	803,404	A→C	intergenic (‑39/+20)	ybhB ← / ← bioA	kinase inhibitor/adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	803,404	0	A	C	100.0%	108.7 / NA	32	intergenic (‑39/+20)	ybhB/bioA	kinase inhibitor/adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/19);  total (13/19)

TGCGGTGAAATATTATCGCCATCGTAACCCATGCCGTTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGT  >  NZ_CP009273/803268‑803521                                                                                                                                         |                                                                                                                      ttctgTGAATTCTTTTCGCCATCGTAACCCATGCCGTTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAccc                                                                                                                     <  2:481971/135‑1 (MQ=255)                                tGCCGTTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCaaaaa                                                                                       >  1:195845/1‑138 (MQ=255)                                tGCCGTTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCaaaaa                                                                                       >  2:414636/1‑138 (MQ=255)                                     ttAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATggt                                                                                 <  1:487533/139‑3 (MQ=255)                                     ttAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGtt                                                                                 <  1:176163/139‑1 (MQ=255)                                     ttAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGtt                                                                                 <  1:244705/139‑1 (MQ=255)                                     ttAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGtt                                                                                 <  1:552250/139‑1 (MQ=255)                                     ttAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGACGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGtt                                                                                 <  1:5684/139‑1 (MQ=255)                                      tAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTTTCAAAAAAATGtt                                                                                 <  2:185641/138‑1 (MQ=255)                                               gACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTa                                                                       >  2:445415/1‑139 (MQ=255)                                               gACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTa                                                                       >  1:384661/1‑139 (MQ=255)                                                    tGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGtt                                                                                 <  1:127561/124‑1 (MQ=255)                                                    tGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGtt                                                                                 >  2:127561/1‑124 (MQ=255)                                                                            aGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGc                                          <  1:65144/139‑1 (MQ=255)                                                                                cGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGc                                       >  2:240217/1‑138 (MQ=255)                                                                                   tACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACt                                   >  1:580682/1‑139 (MQ=255)                                                                                        atgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTg                               <  1:414636/138‑1 (MQ=255)                                                                                        atgaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGc                              >  1:348903/1‑139 (MQ=255)                                                                                          gaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGa                                             <  1:565779/122‑1 (MQ=255)                                                                                          gaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGa                                             >  2:565779/1‑122 (MQ=255)                                                                                          gaGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            >  1:113561/1‑139 (MQ=255)                                                                                           aGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            <  1:390312/138‑1 (MQ=255)                                                                                           aGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            <  2:384661/138‑1 (MQ=255)                                                                                           aGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            <  2:207200/138‑1 (MQ=255)                                                                                           aGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            <  2:195845/138‑1 (MQ=255)                                                                                           aGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            <  1:342303/138‑1 (MQ=255)                                                                                            gTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGga                          <  1:236234/139‑1 (MQ=255)                                                                                             tttCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            >  2:284153/1‑136 (MQ=255)                                                                                             tttCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCgg                            >  1:229753/1‑136 (MQ=255)                                                                                                cATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGaa                       <  1:284153/138‑1 (MQ=255)                                                                                                           ctTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAggg             <  2:25854/137‑1 (MQ=255)                                                                                                           ctTTCTTGTTTGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAggg             <  2:580682/137‑1 (MQ=255)                                                                                                                     tGCAGAAAGTGTAGCCAGAACCCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGt  >  2:581124/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                      TGCGGTGAAATATTATCGCCATCGTAACCCATGCCGTTAAAGACATGACGATGCGGCAATTTATCGCCATCGCGCAGATCGTTACTGATGAGTTTCATGAACCCTCCTTTCTTGTTTGCAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGT  >  NZ_CP009273/803268‑803521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑