Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	803,907	A→G	I269I (ATT→ATC)	bioA ←	adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	803,907	0	A	G	100.0%	38.1 / NA	11	I269I (ATT→ATC)	bioA	adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/5);  total (6/5)

TGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGA  >  NZ_CP009273/803777‑804042                                                                                                                                   |                                                                                                                                        tGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCgg                                                                                                                                 >  1:50400/1‑139 (MQ=255)                                         aCCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTa                                                                                         <  2:50400/139‑1 (MQ=255)                                                   aTGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATc                                                                               >  1:13465/1‑139 (MQ=255)                                                     ggTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATc                                                                               >  1:456959/1‑137 (MQ=255)                                                                     cgcgTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCgg                                                             >  2:147527/1‑139 (MQ=255)                                                                       cgTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCa                                                           <  2:135185/139‑1 (MQ=255)                                                                                                  tgtgCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGAt                                                                     >  1:70946/1‑102 (MQ=255)                                                                                                  tgtgCCGCCGGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGAt                                                                     <  2:70946/102‑1 (MQ=255)                                                                                                        gccgGTTAAGGCTTTACCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTg                          >  2:471066/1‑139 (MQ=255)                                                                                                                       aCCGAGGCACAAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTaa            <  1:383490/138‑1 (MQ=255)                                                                                                                                caAGATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGa  <  2:480556/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCATTCCGGA  >  NZ_CP009273/803777‑804042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑