Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	832,557	T→C	S251P (TCC→CCC)	hcxB →	hydroxycarboxylate dehydrogenase HcXB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	832,557	0	T	C	100.0%	54.2 / NA	17	S251P (TCC→CCC)	hcxB	hydroxycarboxylate dehydrogenase HcXB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

ATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCG  >  NZ_CP009273/832425‑832680                                                                                                                                     |                                                                                                                            aTTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCcgg                                                                                                                         <  2:90022/137‑1 (MQ=255)                gTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCAt                                                                                                         >  1:316130/1‑138 (MQ=255)                 tGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCAt                                                                                                         >  2:505534/1‑137 (MQ=255)                            aaTCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTAc                                                                                            <  1:473747/139‑1 (MQ=255)                                                gTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCAtt                                                                        <  1:443565/139‑1 (MQ=255)                                                               gCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGAcc                                                         <  2:36752/139‑1 (MQ=255)                                                               gCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGAc                                                          <  1:439784/138‑1 (MQ=255)                                                               gCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGAc                                                          >  1:452805/1‑138 (MQ=255)                                                               gCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGAc                                                          <  1:461229/138‑1 (MQ=255)                                                               gCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGAc                                                          <  1:505534/138‑1 (MQ=255)                                                                     gACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTat                                                    <  1:406924/138‑1 (MQ=255)                                                                           tGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTatcatcat                                              >  1:509336/1‑138 (MQ=255)                                                                                             cGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGgc                           >  1:425432/1‑139 (MQ=255)                                                                                                          aTGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                                                               <  2:12720/90‑1 (MQ=255)                                                                                                          aTGTGTGAAATTCTTGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGc                                                               >  1:12720/1‑90 (MQ=255)                                                                                                                       ttGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCg  >  1:459839/1‑138 (MQ=255)                                                                                                                       ttGGCGGGGCGCTTCCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCg  >  2:575185/1‑138 (MQ=255)                                                                                                                                     |                                                                                                                            ATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGCCATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTCTTCGGCGCGCCGGATTGTAACGCGCAGACCG  >  NZ_CP009273/832425‑832680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑