Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	834,220	A→G	D148D (GAT→GAC)	ybiX ←	PKHD‑type hydroxylase YbiX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	834,220	0	A	G	100.0%	90.3 / NA	27	D148D (GAT→GAC)	ybiX	PKHD‑type hydroxylase YbiX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/11);  total (16/11)

ATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGA  >  NZ_CP009273/834104‑834352                                                                                                                     |                                                                                                                                     aTGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTAccc                                                                                                                >  1:355017/1‑139 (MQ=255)          tttttATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTc                                                                                                       >  1:167482/1‑139 (MQ=255)             ttATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCg                                                                                                     <  2:483019/138‑1 (MQ=255)                    cgcgGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTAc                                                                                                                  >  2:424380/1‑118 (MQ=255)                    cgcgGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTAc                                                                                                                  <  1:424380/118‑1 (MQ=255)                    cgcgGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGgtgt                                                                                              >  2:117568/1‑138 (MQ=255)                                               atgatgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcg                                                                    >  1:374534/1‑137 (MQ=255)                                               atgatgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                                  <  1:516493/139‑1 (MQ=255)                                                   tgCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCtt                                                              >  2:334200/1‑139 (MQ=255)                                                    gCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCttt                                                             >  2:355517/1‑139 (MQ=255)                                                     ccACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTtg                                                            >  1:59572/1‑139 (MQ=255)                                                        cTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtg                                                                                                            >  1:286104/1‑88 (MQ=255)                                                        cTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGAtg                                                                                                            <  2:286104/88‑1 (MQ=255)                                                           gTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGAt                                                       <  1:334200/138‑1 (MQ=255)                                                               gccgcGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCtt                                                  <  2:167482/139‑1 (MQ=255)                                                                   cgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcg                                                                    >  2:94626/1‑117 (MQ=255)                                                                   cgGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTaaa                                               >  2:190315/1‑138 (MQ=255)                                                                   cgGGTCACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCcgtcg                                                                    <  1:94626/117‑1 (MQ=255)                                                                                tCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGa                                 <  1:117568/139‑1 (MQ=255)                                                                                       aTGCAGGCTGCTGGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGAGGCAGAAAGGt                            >  2:101971/1‑137 (MQ=255)                                                                                                  tgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                                  <  2:410410/88‑1 (MQ=255)                                                                                                  tgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTa                                                                  >  1:410410/1‑88 (MQ=255)                                                                                                  tgGAGGGATACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATc                 >  2:141085/1‑137 (MQ=255)                                                                                                         aTACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGcc            >  2:209675/1‑135 (MQ=255)                                                                                                         aTACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGcc            <  1:209675/135‑1 (MQ=255)                                                                                                         aTACAACACGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGttt        >  1:5784/1‑139 (MQ=255)                                                                                                               cacGAGGTCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGa  <  1:538442/139‑1 (MQ=255)                                                                                                                     |                                                                                                                                     ATGGCGCGCTTTTTATCATCGCGGATCATCGACTGGATCCACATAAATGATGCCACTCGTACGCCGCGGGTTACGGGTGTCACGCAATGCAGGCTGCTGGAGGGATACAACACGAGATCGCCTGCCGGGAGTTTTACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGA  >  NZ_CP009273/834104‑834352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑