Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	168,561	A→G	T205A (ACG→GCG)	fhuB →	Fe(3+)‑hydroxamate ABC transporter permease FhuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	168,561	0	A	G	100.0%	112.0 / NA	31	T205A (ACG→GCG)	fhuB	Fe(3+)‑hydroxamate ABC transporter permease FhuB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

ACTGGTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTG  >  NZ_CP009273/168437‑168689                                                                                                                             |                                                                                                                                 aCTGGTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACtt                                                                                                                    >  2:230542/1‑139 (MQ=255)     gTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTc                                                                                                                >  1:206999/1‑139 (MQ=255)         tCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGt                                                                                                             >  2:378775/1‑138 (MQ=255)                  atGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGAt                                                                                                   <  2:71562/139‑1 (MQ=255)                       catcTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGc                                                                                              <  2:82645/136‑1 (MQ=255)                         aCTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCtt                                                                                            >  2:334089/1‑139 (MQ=255)                                   aTGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtggcg                                                                                  <  2:512672/139‑1 (MQ=255)                                     gTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtggcgtg                                                                                >  2:40261/1‑139 (MQ=255)                                      tttCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGAtggcgtg                                                                                <  1:378223/138‑1 (MQ=255)                                                 cACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAAtctc                                                                    >  2:348516/1‑139 (MQ=255)                                                      gAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGCGCTTGATGATGGCGTGGCGCGCAATCTCGGGc                                                                <  1:508884/138‑1 (MQ=255)                                                           cTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGc                                                            >  1:486704/1‑137 (MQ=255)                                                           cTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGc                                                            <  2:486704/137‑1 (MQ=255)                                                                        aCTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcg                                                                           >  1:135026/1‑109 (MQ=255)                                                                        aCTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcg                                                                           <  2:135026/109‑1 (MQ=255)                                                                        aCTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGcgcg                                             >  1:173770/1‑139 (MQ=255)                                                                                       aGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGcgcg                                             >  2:393108/1‑124 (MQ=255)                                                                                       aGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGcgcg                                             <  1:393108/124‑1 (MQ=255)                                                                                             tATGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTgg                         <  2:371375/138‑1 (MQ=255)                                                                                              aTGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCg                       >  2:230557/1‑139 (MQ=255)                                                                                               tGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCg                       <  1:40261/138‑1 (MQ=255)                                                                                               tGGCCGCAGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCg                       <  2:520945/138‑1 (MQ=255)                                                                                                      aGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtcat               >  2:356979/1‑139 (MQ=255)                                                                                                      aGCTGCTGGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGtcat               <  1:217486/139‑1 (MQ=255)                                                                                                           ctgGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTg          <  2:206999/139‑1 (MQ=255)                                                                                                           ctgGGCGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTg          <  1:348516/139‑1 (MQ=255)                                                                                                                cGGTGTGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCgctg     <  2:132855/139‑1 (MQ=255)                                                                                                                  gtgtGATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGTTGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCgctgct   >  2:204031/1‑139 (MQ=255)                                                                                                                   tgtgATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGGTGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGctgctg  >  2:75339/1‑139 (MQ=255)                                                                                                                   tgtgATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcg                                                                           <  1:498956/66‑1 (MQ=255)                                                                                                                   tgtgATGCTGGCGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGgcgcg                                                                           >  2:498956/1‑66 (MQ=255)                                                                                                                             |                                                                                                                                 ACTGGTTATCTTCCATCATGACCAACTGCAAAGCATGTTTCTGTGGAGCACTGGAACGCTGACGCAAACCGACTGGGGCGGCGTTGAGCGTTTATGGCCGCAGCTGCTGGGCGGTGTGATGCTGACGTTGCTGCTACTTCGTCCGTTAACCCTGATGGGGCTTGATGATGGCGTGGCGCGCAATCTCGGGCTGGCCTTGTCGCTTGCGCGTCTGGCAGCGCTGTCGCTGGCGATTGTCATCAGTGCGCTGCTG  >  NZ_CP009273/168437‑168689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑