Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	885,786	T→G	L81R (CTG→CGG)	ybjM →	inner membrane protein YbjM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	885,786	0	T	G	100.0%	118.8 / NA	34	L81R (CTG→CGG)	ybjM	inner membrane protein YbjM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/19);  total (15/19)

TCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAA  >  NZ_CP009273/885678‑885914                                                                                                             |                                                                                                                                 tCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     <  1:114284/138‑1 (MQ=255)      cttcCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAagag                                                                                                <  1:353732/138‑1 (MQ=255)             gAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                          <  2:370749/137‑1 (MQ=255)             gAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                          <  1:181973/137‑1 (MQ=255)                   tCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                          <  2:165532/131‑1 (MQ=255)                   tCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                          >  1:165532/1‑131 (MQ=255)                        aGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAgcg                                                                             <  2:123963/139‑1 (MQ=255)                          cttcttTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGcgcg                                                                           <  1:536106/139‑1 (MQ=255)                                    caGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGgt                                                                 <  2:97174/139‑1 (MQ=255)                                       cGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGtgtg                                                              >  2:415115/1‑139 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     <  1:1159/90‑1 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     >  2:1159/1‑90 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                          >  2:76359/1‑101 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCa                                                                                          <  1:76359/101‑1 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTggggg                                                     >  1:124871/1‑138 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTggggg                                                     >  1:496846/1‑138 (MQ=255)                                                 tCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTggggg                                                     >  2:242011/1‑138 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     <  1:147267/88‑1 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     >  2:147267/1‑88 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCCTGGTTACTAAGcgcg                                                                           >  1:174523/1‑114 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGcgcg                                                                           <  2:174523/114‑1 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGgt                                                                 >  1:232531/1‑124 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGgt                                                                 <  2:232531/124‑1 (MQ=255)                                                   cTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGGTACTAAGCGCGGTGTTCTGGTGGGCGCTGGGGGCAc                                                  >  1:163391/1‑139 (MQ=255)                                                      aaaCCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACt                                                 <  2:124871/137‑1 (MQ=255)                                                         ccTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGtt                                            <  2:528249/139‑1 (MQ=255)                                                                         tGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     >  2:133782/1‑66 (MQ=255)                                                                         tGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGc                                                                                                     <  1:133782/66‑1 (MQ=255)                                                                         tGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCagtag                            <  1:415115/139‑1 (MQ=255)                                                                                                   aTTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGGATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGaaaa   >  1:14383/1‑138 (MQ=255)                                                                                                   aTTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGaaaaa  <  2:433155/139‑1 (MQ=255)                                                                                                   aTTATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGaaaa   >  1:182881/1‑138 (MQ=255)                                                                                                     tATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCt                                         <  1:537601/98‑1 (MQ=255)                                                                                                     tATGCGGCGGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCt                                         >  2:537601/1‑98 (MQ=255)                                                                                                             |                                                                                                                                 TCATTCTTCCTGGAGCAGTCGCCAGCTTCTTTTCACAGCGTAGAGAAGTCCTGAAACCTCTGTTTGGCGCAATGCTGGCGGCACCCTGTTCGATGCTCATTATGCGGCTGTTTTTTTCACCGACGCGCTCATTCTGGCAAGAGCTGGCATGGTTACTAAGCGCGGTGTTCTGGTGTGCGCTGGGGGCACTGTGTTTCTTATTTATCAGTAGTTTGTTTAAACCACAGCACAGAAAAA  >  NZ_CP009273/885678‑885914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑