Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	923,801	A→G	V284A (GTC→GCC)	cydC ←	cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	923,801	0	A	G	100.0%	60.5 / NA	17	V284A (GTC→GCC)	cydC	cysteine/glutathione ABC transporter ATP‑binding protein/permease CydC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/7);  total (10/7)

CAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGT  >  NZ_CP009273/923670‑923922                                                                                                                                    |                                                                                                                          cAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAg                                                                                                                    >  2:491764/1‑139 (MQ=255) cAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAg                                                                                                                    >  2:153388/1‑139 (MQ=255)                 cGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGcc                                                                                                     <  2:278747/138‑1 (MQ=255)                                  aGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTgccgcc                                                                                   <  1:281325/139‑1 (MQ=255)                                  aGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTgccgcc                                                                                   <  1:11099/139‑1 (MQ=255)                                             cgtacgGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAAcgccgc                                                                           >  1:307915/1‑136 (MQ=255)                                             cgtacgGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAAcgccgc                                                                           >  2:332912/1‑136 (MQ=255)                                                  ggCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGc                                                                    >  2:458970/1‑138 (MQ=255)                                                                   gCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                  >  1:554309/1‑139 (MQ=255)                                                                   gCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                  >  2:327702/1‑139 (MQ=255)                                                                    ccccAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGAt                                                  <  2:554309/138‑1 (MQ=255)                                                                                     cACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAAt                                >  2:487052/1‑139 (MQ=255)                                                                                             aCTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCa                                  >  2:425907/1‑129 (MQ=255)                                                                                             aCTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCa                                  <  1:425907/129‑1 (MQ=255)                                                                                                    ccAGTGCTTCAAACGCGGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGc                 <  1:491764/139‑1 (MQ=255)                                                                                                                   cgGCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGt  <  1:487052/139‑1 (MQ=255)                                                                                                                     gCTAACGCGCAGAAGGCAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGt  >  2:213536/1‑137 (MQ=255)                                                                                                                                    |                                                                                                                          CAGGAAAGGTGACCTCCGGTTTTTGATCCGTTAAGTCAGAGATACGTACGGCAGAGGCAATGACTTGCCCCAGATGCTGAAATGCACCCGTTACTGGTGCCAGTGCTTCAAACGCGGCTAACGCGCAGAAGACAAACAGGGCAATTAACGCGCCGGGTTGAGCATTGCCGCCAACGCCGCCAGACGCCATCCACAGCATCAGGATCACCGCTAACGCGCCAATGAGCAGCATTATCGCTTGCGACAATGCGGT  >  NZ_CP009273/923670‑923922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑