Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 928,200 T→C R50R (CGT→CGC)  lrp → leucine‑responsive transcriptional regulator Lrp

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273928,2000TC100.0% 133.8 / NA 39R50R (CGT→CGC) lrpleucine‑responsive transcriptional regulator Lrp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

AGAAGCGCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAC  >  NZ_CP009273/928064‑928334
                                                                                                                                        |                                                                                                                                      
agaagCGCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCg                                                                                                                                      <  2:470217/139‑1 (MQ=255)
            gCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACa                                                                                                                          <  2:222862/139‑1 (MQ=255)
                      cGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTAtt                                                                                                                >  1:184852/1‑139 (MQ=255)
                      cGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTAtt                                                                                                                >  2:249528/1‑139 (MQ=255)
                       gACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCTTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTAtt                                                                                                                <  2:179870/138‑1 (MQ=255)
                         ccGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAg                                                                                                             <  2:372658/139‑1 (MQ=255)
                            tATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACa                                                                                                                          <  2:58281/123‑1 (MQ=255)
                            tATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACa                                                                                                                          >  1:58281/1‑123 (MQ=255)
                            tATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGc                                                                                                          >  2:569329/1‑139 (MQ=255)
                                      aaCATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcgc                                                                                                >  1:438040/1‑139 (MQ=255)
                                             ttAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTaa                                                                                         >  1:363812/1‑139 (MQ=255)
                                                     ttGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCAt                                                                                  >  1:22005/1‑138 (MQ=255)
                                                      tGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTa                                                                                >  1:341696/1‑139 (MQ=255)
                                                                  ggCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCAt                                                                     >  2:386952/1‑138 (MQ=255)
                                                                  ggCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCAt                                                                     <  2:341696/138‑1 (MQ=255)
                                                                      tATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACtt                                                                <  2:488300/139‑1 (MQ=255)
                                                                         ttCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACa                                                                                                                          <  2:65328/78‑1 (MQ=255)
                                                                         ttCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACa                                                                                                                          >  1:65328/1‑78 (MQ=255)
                                                                                 tCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGt                                                     <  1:426465/139‑1 (MQ=255)
                                                                                      cTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTgaga                                                <  2:22005/139‑1 (MQ=255)
                                                                                      cTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTGGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACGTCTGGTATTCGTTgaga                                                <  1:339545/139‑1 (MQ=255)
                                                                                          cTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTAc                                            >  1:403852/1‑139 (MQ=255)
                                                                                             aaCGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTAct                                           <  1:510464/137‑1 (MQ=255)
                                                                                                   tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     >  1:107340/1‑57 (MQ=255)
                                                                                                   tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGt                                                                                                                     <  2:107340/57‑1 (MQ=255)
                                                                                                   tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAggg                                                                                                           >  2:317726/1‑67 (MQ=255)
                                                                                                   tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAggg                                                                                                           <  1:317726/67‑1 (MQ=255)
                                                                                                   tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTa                                                                                <  1:30829/94‑1 (MQ=255)
                                                                                                   tgGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTa                                                                                >  2:30829/1‑94 (MQ=255)
                                                                                                      gACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTgg                                <  2:438040/139‑1 (MQ=255)
                                                                                                            ccccAACGCCGTGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCAcc                          >  2:506078/1‑139 (MQ=255)
                                                                                                                       tGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcg                                                                                                 <  1:530670/57‑1 (MQ=255)
                                                                                                                       tGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGgcg                                                                                                 >  2:530670/1‑57 (MQ=255)
                                                                                                                       tGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGttt                >  2:508615/1‑138 (MQ=255)
                                                                                                                       tGCCTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGttt                >  1:475261/1‑138 (MQ=255)
                                                                                                                         ccTTGAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTCCTCTGAATCGTGGCGCACCGGATGTGTTTGaa             <  1:508615/139‑1 (MQ=255)
                                                                                                                             gAGCGTGTGCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACaa          <  1:249528/138‑1 (MQ=255)
                                                                                                                                    tgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAc  >  2:494534/1‑139 (MQ=255)
                                                                                                                                    tgCGCCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAc  <  2:475261/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AGAAGCGCCCTGGCAAAGATCTCGACCGTATCGATCGTAACATTCTTAATGAGTTGCAAAAGGATGGGCGTATTTCTAACGTCGAGCTTTCTAAACGTGTGGGACTTTCCCCAACGCCGTGCCTTGAGCGTGTGCGTCGGCTGGAAAGACAAGGGTTTATTCAGGGCTATACGGCGCTGCTTAACCCCCATTATCTGGATGCATCACTTCTGGTATTCGTTGAGATTACTCTGAATCGTGGCGCACCGGATGTGTTTGAACAATTCAATAC  >  NZ_CP009273/928064‑928334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑