Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	960,653	T→C	M293T (ATG→ACG)	ycaI →	ComEC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	960,653	0	T	C	100.0%	134.4 / NA	41	M293T (ATG→ACG)	ycaI	ComEC family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/23);  total (18/23)

TTTCTGCCTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAATGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTCGCGGCATTG  >  NZ_CP009273/960520‑960786                                                                                                                                      |                                                                                                                                      tttCTGCCTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCtt                                                                                                                                   <  1:544296/138‑1 (MQ=255)        cTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCttaagttaag                                                                                                                           >  1:318959/1‑139 (MQ=255)            gcgcTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTggg                                                                                                                       <  2:569459/139‑1 (MQ=255)            gcgcTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTggg                                                                                                                       <  1:234853/139‑1 (MQ=255)                      cACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtgga                                                                                                             <  2:119981/139‑1 (MQ=255)                       aCTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                            <  1:48571/139‑1 (MQ=255)                            cAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCt                                                                                                       >  2:430287/1‑139 (MQ=255)                               aTACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                                    <  2:520644/139‑1 (MQ=255)                               aTACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                                    <  2:318959/139‑1 (MQ=255)                               aTACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                                    <  1:486750/139‑1 (MQ=255)                                     ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                            <  1:21486/125‑1 (MQ=255)                                     ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                            >  2:21486/1‑125 (MQ=255)                                     ttAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTAt                                                                                              >  1:275313/1‑139 (MQ=255)                                         ttGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGat                                                                                          <  1:344037/139‑1 (MQ=255)                                             cGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtgga                                                                                                             >  1:108298/1‑116 (MQ=255)                                             cGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtgga                                                                                                             <  2:108298/116‑1 (MQ=255)                                                  tCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                            <  1:382048/112‑1 (MQ=255)                                                  tCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACagtggag                                                                                                            >  2:382048/1‑112 (MQ=255)                                                       tgtgctgCTTTTTATGCCTGGTTGACGGGAACGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTggcgg                                                                            >  2:322843/1‑139 (MQ=255)                                                              ctTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAAtttt                                                                     >  2:477901/1‑139 (MQ=255)                                                                       ccTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGa                                                            <  1:477901/139‑1 (MQ=255)                                                                          ggTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                                    >  2:327109/1‑96 (MQ=255)                                                                          ggTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTggg                                                                                                    <  1:327109/96‑1 (MQ=255)                                                                           gTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTCTGTGGGGAACGCTTAAGTTACGTGGGCGACAGAGGAGTGGCTGGGATGTATGGATAGGTTGTCTGGCGGCAATTTTGCTGATGGATccg                                                        >  1:282897/1‑138 (MQ=255)                                                                            ttGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTg                                                       <  2:135544/139‑1 (MQ=255)                                                                                            ctcctGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCa                                       <  2:48811/139‑1 (MQ=255)                                                                                                  cATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGtt                                  >  1:529221/1‑138 (MQ=255)                                                                                                     tGCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATAGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATg                              >  2:289348/1‑139 (MQ=255)                                                                                                      gCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGat                                                                                          <  1:323028/78‑1 (MQ=255)                                                                                                      gCGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGat                                                                                          >  2:323028/1‑78 (MQ=255)                                                                                                       cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGc                            >  2:440840/1‑139 (MQ=255)                                                                                                       cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGc                            >  1:39989/1‑139 (MQ=255)                                                                                                       cGTACCATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGc                            >  2:180923/1‑139 (MQ=255)                                                                                                           ccATGGTGGCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGATAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGctct                         <  1:302364/138‑1 (MQ=255)                                                                                                              tggtggCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGc                     >  2:270527/1‑139 (MQ=255)                                                                                                                 tggCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCt                                                                  >  1:492506/1‑91 (MQ=255)                                                                                                                 tggCGCTTGCTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCt                                                                  <  2:492506/91‑1 (MQ=255)                                                                                                                          caaCGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGAGATGTATGGATATGTTGTCTGGCAGCAATTTTGCTAATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCACTGCGGTcg          <  2:282897/136‑1 (MQ=255)                                                                                                                          cTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTcg          <  2:529221/138‑1 (MQ=255)                                                                                                                          cTACGTGGGGAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTcg          <  1:373583/138‑1 (MQ=255)                                                                                                                                 gggAACGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTCGCGGCATTg  <  1:270527/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTTCTGCCTGGGCGCTGGATCCACTGGCAAATACCATTAATTGGCGGAATCTGCTGTGCTGCTTTTTATGCCTGGTTGACGGGAATGCAACCTCCTGCATTGCGTACCATGGTGGCGCTTGCTACGTGGGGAATGCTTAAGTTAAGTGGGCGACAGTGGAGTGGCTGGGATGTATGGATATGTTGTCTGGCGGCAATTTTGCTGATGGATCCTGTTGCCATTCTCTCGCAAAGTTTATGGCTCTCTGCCGCTGCGGTCGCGGCATTG  >  NZ_CP009273/960520‑960786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑