Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	974,173	T→C	S798P (TCC→CCC)	mukB →	chromosome partition protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	974,173	0	T	C	100.0%	83.1 / NA	24	S798P (TCC→CCC)	mukB	chromosome partition protein MukB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/13);  total (11/13)

GCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACT  >  NZ_CP009273/974040‑974303                                                                                                                                      |                                                                                                                                   gCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGaa                                                                                                                               <  1:415840/139‑1 (MQ=255)        aaaGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCg                                                                                                                        <  1:161272/139‑1 (MQ=255)            cGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt                                                                                                                           >  2:482092/1‑132 (MQ=255)            cGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCt                                                                                                                           <  1:482092/132‑1 (MQ=255)               tAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct                                                                                                                 <  1:205802/139‑1 (MQ=255)               tAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct                                                                                                                 <  1:394111/139‑1 (MQ=255)                    gtgAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCa                                                                                                                     >  2:506736/1‑130 (MQ=255)                    gtgAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCa                                                                                                                     <  1:506736/130‑1 (MQ=255)                       aaaaTCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTg                                                                                                         <  2:124102/139‑1 (MQ=255)                           tCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGt                                                                                                     <  2:431151/139‑1 (MQ=255)                                           gCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGt                                                                                     <  2:303340/139‑1 (MQ=255)                                              ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg                                                                                                                   >  1:359646/1‑106 (MQ=255)                                              ttATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACg                                                                                                                   <  2:359646/106‑1 (MQ=255)                                                aTTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCa                                                                                >  1:329670/1‑139 (MQ=255)                                                      gTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGc                                                                          >  1:270565/1‑139 (MQ=255)                                                           ccGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct                                                                                                                 <  2:270016/95‑1 (MQ=255)                                                           ccGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGct                                                                                                                 >  1:270016/1‑95 (MQ=255)                                                                             ggTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGt                                                    <  2:425927/138‑1 (MQ=255)                                                                                  tgctgcGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTg                                              >  2:376371/1‑139 (MQ=255)                                                                                         cgTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTg                                       >  1:225158/1‑139 (MQ=255)                                                                                                                ccATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGt                              <  1:448317/125‑1 (MQ=255)                                                                                                                ccATGCCGAGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGt                              >  2:448317/1‑125 (MQ=255)                                                                                                                        aGCGTGAAGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCg        >  2:550356/1‑139 (MQ=255)                                                                                                                               aGTGCTTCCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACt  >  2:318141/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                   GCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAGGCGTTCAGCCGCTTTATCGGCAGTCATCTGGCGGTTGCGTTTGAGTCTGACCCGGAAGCAGAAATCCGTCAACT  >  NZ_CP009273/974040‑974303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑