Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	977,344	A→G	D281G (GAC→GGC)	ldtD →	L,D‑transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	977,344	0	A	G	100.0%	66.6 / NA	19	D281G (GAC→GGC)	ldtD	L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/11);  total (8/11)

CGCCCAGGGCAGTGGAGTAACGACGTACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTCGCGCCGCCTACGATA  >  NZ_CP009273/977232‑977457                                                                                                                 |                                                                                                                  cgcCCAGGGCAGTGGAGTAACGACGTACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGtt                                                                                          <  2:413671/138‑1 (MQ=255)               gAGTAACGACGTACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAAcagca                                                                           <  1:328618/139‑1 (MQ=255)                       acgTACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACt                                                                     >  1:249606/1‑137 (MQ=255)                          tACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGcc                                                                >  1:81284/1‑139 (MQ=255)                          tACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCAATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGcc                                                                >  2:579357/1‑139 (MQ=255)                                              ttGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGGTGGATAAGCAAACGACGt                                            <  1:232553/139‑1 (MQ=255)                                                 cAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGtct                                          <  2:249606/138‑1 (MQ=255)                                                    cGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCg                                        >  2:545354/1‑137 (MQ=255)                                                    cGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCg                                        >  2:175493/1‑137 (MQ=255)                                                    cGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCg                                        >  2:245430/1‑137 (MQ=255)                                                     gCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCgtag                                     <  1:245430/139‑1 (MQ=255)                                                                         gggCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTc                 >  1:579069/1‑139 (MQ=255)                                                                          ggCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCg                                                               <  2:454429/92‑1 (MQ=255)                                                                          ggCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCg                                                               >  1:454429/1‑92 (MQ=255)                                                                            ccGAAAATTCCTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTcgcg              <  2:241210/139‑1 (MQ=255)                                                                            ccGAAAATTACTCTCCCTGGCGATGACACGCCAACTGGCGCGGTAGGCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTcgcg              <  2:389522/139‑1 (MQ=255)                                                                            ccGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTcgcg              <  1:545354/139‑1 (MQ=255)                                                                            ccGAAAATTACTCTACCTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTcgcg              <  1:501537/139‑1 (MQ=255)                                                                                           ccTGGCGATGACACGCCAACTGGCGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGAGGGATAAGCAAACGACGTCTCGTAGTAAACCGGCGCCTGCCGTTCGCGCCGCCTACGATa  <  2:81284/136‑1 (MQ=255)                                                                                                                 |                                                                                                                  CGCCCAGGGCAGTGGAGTAACGACGTACCGGCGTTGCGCGAAATATTGCAACGCACAGGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCGTTCGCGCCGCCTACGATA  >  NZ_CP009273/977232‑977457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑